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10.1111/jpc.15065 http://scihub22266oqcxt.onion/10.1111/jpc.15065 32767639!7436299!32767639     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Paediatr+Child+Health 2020 ; 56 (12): 1872-1874 Nephropedia Template TP {{tp|p=32767639|t=2020. SARS-CoV-2 in human milk is inactivated by Holder pasteurisation but not cold storage |pdf=|usr=}}{{32767639}} gab.com Text SARS-CoV-2 in human milk is inactivated by Holder pasteurisation but not cold storage JO: J Paediatr Child Health http://www.kidney.de/pget.php?&tw=1&pmid=32767639 #FOAvip AIM: As the COVID-19 pandemic evolves, human milk banks world-wide continue to provide donor human milk to vulnerable infants who lack access to mother's own milk. Under these circumstances, ensuring the safety of donor human milk is paramount, as the risk of vertical transmission of SARS-CoV-2 is not fully understood. Here, we investigate the inactivation of SARS-CoV-2 in human milk by pasteurisation and the stability of SARS-CoV-2 in human milk under cold storage. METHODS: SARS-CoV-2 was experimentally inoculated into human milk samples from healthy donors or into a control medium. Triplicates of each sample were layered onto uninfected cells after Holder pasteurisation (63 degrees C for 30 min), heating to 56 degrees C for 30 min, or after 48 h of storage at 4 degrees C or -30 degrees C. Infectious titres of virus were determined at 72 h post-infection by endpoint titration. RESULTS: Following heating to 63 degrees C or 56 degrees C for 30 min, replication competent (i.e. live) SARS-CoV-2 was undetected in both human milk and the control medium. Cold storage of SARS-CoV-2 in human milk (either at 4 degrees C or -30 degrees C) did not significantly impact infectious viral load over a 48 h period. CONCLUSION: SARS-CoV-2 is effectively inactivated by Holder pasteurisation, suggesting that existing milk bank processes will effectively mitigate the risk of transmission of SARS-COV-2 to vulnerable infants through pasteurised donor human milk. The demonstrated stability of SARS-CoV-2 in refrigerated or frozen human milk may assist in the development of guidelines around safe expressing and storing of milk from COVID-19 infected mothers. Twit Text FOAVip SARS-CoV-2 in human milk is inactivated by Holder pasteurisation but not cold storage ????J Paediatr Child Health http://www.kidney.de/pget.php?&tw=1&pmid=32767639 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32767639 #FOAvip English Wikipedia <ref>{{Cite pmid|32767639}}</ref> SARS-CoV-2 in human milk is inactivated by Holder pasteurisation but not cold storage #MMPMID32767639 Walker GJ; Clifford V; Bansal N; Stella AO; Turville S; Stelzer-Braid S; Klein LD; Rawlinson W J Paediatr Child Health 2020[Dec]; 56 (12): 1872-1874 PMID32767639show ga AIM: As the COVID-19 pandemic evolves, human milk banks world-wide continue to provide donor human milk to vulnerable infants who lack access to mother's own milk. Under these circumstances, ensuring the safety of donor human milk is paramount, as the risk of vertical transmission of SARS-CoV-2 is not fully understood. Here, we investigate the inactivation of SARS-CoV-2 in human milk by pasteurisation and the stability of SARS-CoV-2 in human milk under cold storage. METHODS: SARS-CoV-2 was experimentally inoculated into human milk samples from healthy donors or into a control medium. Triplicates of each sample were layered onto uninfected cells after Holder pasteurisation (63 degrees C for 30 min), heating to 56 degrees C for 30 min, or after 48 h of storage at 4 degrees C or -30 degrees C. Infectious titres of virus were determined at 72 h post-infection by endpoint titration. RESULTS: Following heating to 63 degrees C or 56 degrees C for 30 min, replication competent (i.e. live) SARS-CoV-2 was undetected in both human milk and the control medium. Cold storage of SARS-CoV-2 in human milk (either at 4 degrees C or -30 degrees C) did not significantly impact infectious viral load over a 48 h period. CONCLUSION: SARS-CoV-2 is effectively inactivated by Holder pasteurisation, suggesting that existing milk bank processes will effectively mitigate the risk of transmission of SARS-COV-2 to vulnerable infants through pasteurised donor human milk. The demonstrated stability of SARS-CoV-2 in refrigerated or frozen human milk may assist in the development of guidelines around safe expressing and storing of milk from COVID-19 infected mothers. |*COVID-19[MESH] |*Cold Temperature[MESH] |*Pasteurization[MESH] |*SARS-CoV-2[MESH] |*Virus Inactivation[MESH] |Humans[MESH]SARS-CoV-2 in human milk is inactivated by Holder pasteurisation but n(epmc).pdf DeepDyve Pubget Overpricing