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10.1007/s11596-020-2224-y http://scihub22266oqcxt.onion/10.1007/s11596-020-2224-y 32767258!7412769!32767258     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Curr+Med+Sci 2020 ; 40 (4): 614-617 Nephropedia Template TP {{tp|p=32767258|t=2020. Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses |pdf=|usr=}}{{32767258}} gab.com Text Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses JO: Curr Med Sci http://www.kidney.de/pget.php?&tw=1&pmid=32767258 #FOAvip The novel Coronavirus SARS-CoV-2 caused an outbreak of pneumonia in Wuhan, Hubei province of China in January 2020. This study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for SARS-CoV-2. Twelve patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13, 2020 and throat swabs were taken. The swabs were stored at room temperature (20-25 degrees C), then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses (56 degrees C for 30, 45, 60 min; 65, 70, 80 degrees C for 10, 15, 20 min). Control aliquots were stored at room temperature for 60 min. Then all aliquots were tested in a real-time fluorescence PCR using primers against SARS-CoV-2. Regardless of inactivation temperature and time, 7 of 12 cases (58.3%) tested were positive for SARS-CoV-2 by PCR, and cycle threshold values were similar. These results suggest that virus inactivation parameters exert minimal influence on PCR test results. Inactivation at 65 degrees C for 10 min may be sufficient to ensure safe, reliable testing. Twit Text FOAVip Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses ????Curr Med Sci http://www.kidney.de/pget.php?&tw=1&pmid=32767258 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32767258 #FOAvip English Wikipedia <ref>{{Cite pmid|32767258}}</ref> Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses #MMPMID32767258 Wu ZG; Zheng HY; Gu J; Li F; Lv RL; Deng YY; Xu WZ; Tong YQ Curr Med Sci 2020[Aug]; 40 (4): 614-617 PMID32767258show ga The novel Coronavirus SARS-CoV-2 caused an outbreak of pneumonia in Wuhan, Hubei province of China in January 2020. This study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for SARS-CoV-2. Twelve patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13, 2020 and throat swabs were taken. The swabs were stored at room temperature (20-25 degrees C), then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses (56 degrees C for 30, 45, 60 min; 65, 70, 80 degrees C for 10, 15, 20 min). Control aliquots were stored at room temperature for 60 min. Then all aliquots were tested in a real-time fluorescence PCR using primers against SARS-CoV-2. Regardless of inactivation temperature and time, 7 of 12 cases (58.3%) tested were positive for SARS-CoV-2 by PCR, and cycle threshold values were similar. These results suggest that virus inactivation parameters exert minimal influence on PCR test results. Inactivation at 65 degrees C for 10 min may be sufficient to ensure safe, reliable testing. |*Virus Inactivation[MESH] |Adult[MESH] |Aged[MESH] |Betacoronavirus/*genetics/*isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |China/epidemiology[MESH] |Clinical Laboratory Techniques/*methods[MESH] |Coronavirus Infections/*diagnosis/epidemiology/*virology[MESH] |Humans[MESH] |Infection Control/methods[MESH] |Medical Laboratory Personnel[MESH] |Middle Aged[MESH] |Molecular Diagnostic Techniques/methods[MESH] |Occupational Exposure/prevention & control[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/epidemiology/*virology[MESH] |RNA, Viral/analysis/genetics[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2[MESH] |Temperature[MESH]Effects of Different Temperature and Time Durations of Virus Inactivat(epmc).pdf DeepDyve Pubget Overpricing