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10.1016/j.bios.2020.112479 http://scihub22266oqcxt.onion/10.1016/j.bios.2020.112479 32763826!7387931!32763826     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2020 ; 167 (ä): 112479 Nephropedia Template TP {{tp|p=32763826|t=2020. DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis |pdf=|usr=}}{{32763826}} gab.com Text DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=32763826 #FOAvip COVID-19 pandemic outbreak is the most astounding scene ever experienced in the 21st century. It has been determined to be caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). With the global pandemic, the lack of efficient rapid and accurate molecular diagnostic testing tools has hindered the public opportunely response to the emerging viral threat. Herein, a DNA nanoscaffold hybrid chain reaction (DNHCR)-based nucleic acid assay strategy is reported for rapid detection of SARS-CoV-2 RNA. In this method, the DNA nanoscaffolds have been first constructed by the self-assembly of long DNA strands and self-quenching probes (H1). Then, the SARS-CoV-2 RNA will initiate the hybridization of H1 and free H2 DNA probes along the nanoscaffold, and an illuminated DNA nanostring is instantly obtained. By taking advantages of the localization design of the H1 probes and the temperature tolerance of the isothermal amplification, the proposed DNHCR method can detect target at short responding time (within 10 min) and mild condition (15 degrees C-35 degrees C). Moreover, the reliability of DNHCR method in serum and saliva samples have also been validated. Therefore, DNHCR-based method is expected to provide a simple and faster alternative to the traditional SARS-CoV-2 qRT-PCR assay. Twit Text FOAVip DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=32763826 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32763826 #FOAvip English Wikipedia <ref>{{Cite pmid|32763826}}</ref> DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis #MMPMID32763826 Jiao J; Duan C; Xue L; Liu Y; Sun W; Xiang Y Biosens Bioelectron 2020[Nov]; 167 (ä): 112479 PMID32763826show ga COVID-19 pandemic outbreak is the most astounding scene ever experienced in the 21st century. It has been determined to be caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). With the global pandemic, the lack of efficient rapid and accurate molecular diagnostic testing tools has hindered the public opportunely response to the emerging viral threat. Herein, a DNA nanoscaffold hybrid chain reaction (DNHCR)-based nucleic acid assay strategy is reported for rapid detection of SARS-CoV-2 RNA. In this method, the DNA nanoscaffolds have been first constructed by the self-assembly of long DNA strands and self-quenching probes (H1). Then, the SARS-CoV-2 RNA will initiate the hybridization of H1 and free H2 DNA probes along the nanoscaffold, and an illuminated DNA nanostring is instantly obtained. By taking advantages of the localization design of the H1 probes and the temperature tolerance of the isothermal amplification, the proposed DNHCR method can detect target at short responding time (within 10 min) and mild condition (15 degrees C-35 degrees C). Moreover, the reliability of DNHCR method in serum and saliva samples have also been validated. Therefore, DNHCR-based method is expected to provide a simple and faster alternative to the traditional SARS-CoV-2 qRT-PCR assay. |*Betacoronavirus/genetics/isolation & purification[MESH] |Biosensing Techniques/*methods[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/*methods/statistics & numerical data[MESH] |Coronavirus Infections/*diagnosis/epidemiology/*virology[MESH] |DNA/chemical synthesis/chemistry/genetics[MESH] |Feasibility Studies[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/methods/statistics & numerical data[MESH] |Nanostructures/chemistry[MESH] |Nanotechnology[MESH] |Nucleic Acid Amplification Techniques/methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/epidemiology/*virology[MESH] |RNA, Viral/analysis/genetics[MESH] |Reproducibility of Results[MESH] |SARS-CoV-2[MESH]DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis (epmc).pdf DeepDyve Pubget Overpricing