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Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Arch+Virol 2020 ; 165 (10): 2373-2377 Nephropedia Template TP
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Detection of SARS-CoV-2 by RNAscope((R)) in situ hybridization and immunohistochemistry techniques #MMPMID32761270
Carossino M; Ip HS; Richt JA; Shultz K; Harper K; Loynachan AT; Del Piero F; Balasuriya UBR
Arch Virol 2020[Oct]; 165 (10): 2373-2377 PMID32761270show ga
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope((R))-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.