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10.3390/v12080849

http://scihub22266oqcxt.onion/10.3390/v12080849
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32759673!7472307!32759673
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suck abstract from ncbi


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pmid32759673      Viruses 2020 ; 12 (8): ä
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  • Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by Mass Spectrometry #MMPMID32759673
  • Wandernoth P; Kriegsmann K; Groh-Mohanu C; Daeumer M; Gohl P; Harzer O; Kriegsmann M; Kriegsmann J
  • Viruses 2020[Aug]; 12 (8): ä PMID32759673show ga
  • BACKGROUND: Amplification of viral ribonucleic acid (RNA) by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the initial outbreak, strategies to detect and isolate patients have been important to avoid uncontrolled viral spread. Although testing capacities have been upscaled, there is still a need for reliable high throughput test systems, specifically those that require alternative consumables. Therefore, we tested and compared two different methods for the detection of viral PCR products: rRT-PCR and mass spectrometry (MS). METHODS: Viral RNA was isolated and amplified from oro- or nasopharyngeal swabs. A total of 22 samples that tested positive and 22 samples that tested negative for SARS-CoV-2 by rRT-PCR were analyzed by MS. Results of the rRT-PCR and the MS protocol were compared. RESULTS: Results of rRT-PCR and the MS test system were in concordance in all samples. Time-to-results was faster for rRT-PCR. Hands-on-time was comparable in both assays. CONCLUSIONS: MS is a fast, reliable and cost-effective alternative for the detection of SARS-CoV-2 from oral and nasopharyngeal swabs.
  • |Adolescent[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Betacoronavirus/*isolation & purification[MESH]
  • |COVID-19[MESH]
  • |Child[MESH]
  • |Coronavirus Infections/*diagnosis/virology[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Mass Spectrometry/*methods[MESH]
  • |Middle Aged[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/virology[MESH]
  • |RNA, Viral/analysis[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2[MESH]
  • |Time Factors[MESH]


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