Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1172/JCI141374 http://scihub22266oqcxt.onion/10.1172/JCI141374 32759504!7598040!32759504     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Invest 2020 ; 130 (11): 6151-6157 Nephropedia Template TP {{tp|p=32759504|t=2020. Complement and tissue factor-enriched neutrophil extracellular traps are key drivers in COVID-19 immunothrombosis |pdf=|usr=}}{{32759504}} gab.com Text Complement and tissue factor-enriched neutrophil extracellular traps are key drivers in COVID-19 immunothrombosis JO: J Clin Invest http://www.kidney.de/pget.php?&tw=1&pmid=32759504 #FOAvip Emerging data indicate that complement and neutrophils contribute to the maladaptive immune response that fuels hyperinflammation and thrombotic microangiopathy, thereby increasing coronavirus 2019 (COVID-19) mortality. Here, we investigated how complement interacts with the platelet/neutrophil extracellular traps (NETs)/thrombin axis, using COVID-19 specimens, cell-based inhibition studies, and NET/human aortic endothelial cell (HAEC) cocultures. Increased plasma levels of NETs, tissue factor (TF) activity, and sC5b-9 were detected in patients. Neutrophils of patients yielded high TF expression and released NETs carrying active TF. Treatment of control neutrophils with COVID-19 platelet-rich plasma generated TF-bearing NETs that induced thrombotic activity of HAECs. Thrombin or NETosis inhibition or C5aR1 blockade attenuated platelet-mediated NET-driven thrombogenicity. COVID-19 serum induced complement activation in vitro, consistent with high complement activity in clinical samples. Complement C3 inhibition with compstatin Cp40 disrupted TF expression in neutrophils. In conclusion, we provide a mechanistic basis for a pivotal role of complement and NETs in COVID-19 immunothrombosis. This study supports strategies against severe acute respiratory syndrome coronavirus 2 that exploit complement or NETosis inhibition. Twit Text FOAVip Complement and tissue factor-enriched neutrophil extracellular traps are key drivers in COVID-19 immunothrombosis ????J Clin Invest http://www.kidney.de/pget.php?&tw=1&pmid=32759504 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32759504 #FOAvip English Wikipedia <ref>{{Cite pmid|32759504}}</ref> Complement and tissue factor-enriched neutrophil extracellular traps are key drivers in COVID-19 immunothrombosis #MMPMID32759504 Skendros P; Mitsios A; Chrysanthopoulou A; Mastellos DC; Metallidis S; Rafailidis P; Ntinopoulou M; Sertaridou E; Tsironidou V; Tsigalou C; Tektonidou M; Konstantinidis T; Papagoras C; Mitroulis I; Germanidis G; Lambris JD; Ritis K J Clin Invest 2020[Nov]; 130 (11): 6151-6157 PMID32759504show ga Emerging data indicate that complement and neutrophils contribute to the maladaptive immune response that fuels hyperinflammation and thrombotic microangiopathy, thereby increasing coronavirus 2019 (COVID-19) mortality. Here, we investigated how complement interacts with the platelet/neutrophil extracellular traps (NETs)/thrombin axis, using COVID-19 specimens, cell-based inhibition studies, and NET/human aortic endothelial cell (HAEC) cocultures. Increased plasma levels of NETs, tissue factor (TF) activity, and sC5b-9 were detected in patients. Neutrophils of patients yielded high TF expression and released NETs carrying active TF. Treatment of control neutrophils with COVID-19 platelet-rich plasma generated TF-bearing NETs that induced thrombotic activity of HAECs. Thrombin or NETosis inhibition or C5aR1 blockade attenuated platelet-mediated NET-driven thrombogenicity. COVID-19 serum induced complement activation in vitro, consistent with high complement activity in clinical samples. Complement C3 inhibition with compstatin Cp40 disrupted TF expression in neutrophils. In conclusion, we provide a mechanistic basis for a pivotal role of complement and NETs in COVID-19 immunothrombosis. This study supports strategies against severe acute respiratory syndrome coronavirus 2 that exploit complement or NETosis inhibition. |*Betacoronavirus/immunology/metabolism[MESH] |*Complement Membrane Attack Complex/immunology/metabolism[MESH] |*Coronavirus Infections/blood/immunology[MESH] |*Extracellular Traps/immunology/metabolism[MESH] |*Neutrophils/immunology/metabolism[MESH] |*Pandemics[MESH] |*Pneumonia, Viral/blood/immunology[MESH] |*Thromboplastin/immunology/metabolism[MESH] |*Thrombosis/blood/immunology/virology[MESH] |Aged[MESH] |COVID-19[MESH] |Complement Activation/drug effects[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Peptides, Cyclic/pharmacology[MESH] |Receptor, Anaphylatoxin C5a/antagonists & inhibitors/blood/immunology[MESH] |Respiratory Distress Syndrome/blood/immunology/virology[MESH] |SARS-CoV-2[MESH]Complement and tissue factor-enriched neutrophil extracellular traps a(epmc).pdf DeepDyve Pubget Overpricing