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10.1016/j.scitotenv.2020.140621 http://scihub22266oqcxt.onion/10.1016/j.scitotenv.2020.140621 32758821!7833249!32758821     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Total+Environ 2020 ; 743 (ä): 140621 Nephropedia Template TP {{tp|p=32758821|t=2020. First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA |pdf=|usr=}}{{32758821}} gab.com Text First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA JO: Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=32758821 #FOAvip We investigated the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples in southern Louisiana, USA. Untreated and treated wastewater samples were collected on five occasions over a four-month period from January to April 2020. The wastewater samples were concentrated via ultrafiltration (Method A), and an adsorption-elution method using electronegative membranes (Method B). SARS-CoV-2 RNA was detected in 2 out of 15 wastewater samples using two reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays (CDC N1 and N2). None of the secondary treated and final effluent samples tested positive for SARS-CoV-2 RNA. To our knowledge, this is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in North America, including the USA. However, concentration methods and RT-qPCR assays need to be refined and validated to increase the sensitivity of SARS-CoV-2 RNA detection in wastewater. Twit Text FOAVip First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA ????Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=32758821 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32758821 #FOAvip English Wikipedia <ref>{{Cite pmid|32758821}}</ref> First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA #MMPMID32758821 Sherchan SP; Shahin S; Ward LM; Tandukar S; Aw TG; Schmitz B; Ahmed W; Kitajima M Sci Total Environ 2020[Nov]; 743 (ä): 140621 PMID32758821show ga We investigated the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples in southern Louisiana, USA. Untreated and treated wastewater samples were collected on five occasions over a four-month period from January to April 2020. The wastewater samples were concentrated via ultrafiltration (Method A), and an adsorption-elution method using electronegative membranes (Method B). SARS-CoV-2 RNA was detected in 2 out of 15 wastewater samples using two reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays (CDC N1 and N2). None of the secondary treated and final effluent samples tested positive for SARS-CoV-2 RNA. To our knowledge, this is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in North America, including the USA. However, concentration methods and RT-qPCR assays need to be refined and validated to increase the sensitivity of SARS-CoV-2 RNA detection in wastewater. |*Coronavirus Infections[MESH] |*Pandemics[MESH] |*Pneumonia, Viral[MESH] |*RNA[MESH] |*Wastewater[MESH] |Betacoronavirus[MESH] |COVID-19[MESH] |Humans[MESH] |Louisiana[MESH] |North America[MESH]First detection of SARS-CoV-2 RNA in wastewater in North America: A st(epmc).pdf DeepDyve Pubget Overpricing