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10.1186/s12917-020-02494-1 http://scihub22266oqcxt.onion/10.1186/s12917-020-02494-1 32758221!7404080!32758221     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Vet+Res 2020 ; 16 (1): 272 Nephropedia Template TP {{tp|p=32758221|t=2020. First report and genetic characterization of bovine torovirus in diarrhoeic calves in China |pdf=|usr=}}{{32758221}} gab.com Text First report and genetic characterization of bovine torovirus in diarrhoeic calves in China JO: BMC Vet Res http://www.kidney.de/pget.php?&tw=1&pmid=32758221 #FOAvip BACKGROUND: Coronaviruses are notorious pathogens that cause diarrheic and respiratory diseases in humans and animals. Although the epidemiology and pathogenicity of coronaviruses have gained substantial attention, little is known about bovine coronavirus in cattle, which possesses a close relationship with human coronavirus. Bovine torovirus (BToV) is a newly identified relevant pathogen associated with cattle diarrhoea and respiratory diseases, and its epidemiology in the Chinese cattle industry remains unknown. RESULTS: In this study, a total of 461 diarrhoeic faecal samples were collected from 38 different farms in three intensive cattle farming regions and analysed. Our results demonstrated that BToV is present in China, with a low prevalence rate of 1.74% (8/461). The full-length spike genes were further cloned from eight clinical samples (five farms in Henan Province). Phylogenetic analysis showed that two different subclades of BToV strains are circulating in China. Meanwhile, the three BToV strains identified from dairy calves, 18,307, 2YY and 5YY, all contained the amino acid variants R614Q, I801T, N841S and Q885E. CONCLUSIONS: This is the first report to confirm the presence of BToV in beef and dairy calves in China with diarrhea, which extend our understanding of the epidemiology of BToVs worldwide. Twit Text FOAVip First report and genetic characterization of bovine torovirus in diarrhoeic calves in China ????BMC Vet Res http://www.kidney.de/pget.php?&tw=1&pmid=32758221 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32758221 #FOAvip English Wikipedia <ref>{{Cite pmid|32758221}}</ref> First report and genetic characterization of bovine torovirus in diarrhoeic calves in China #MMPMID32758221 Shi Z; Wang W; Chen C; Zhang X; Wang J; Xu Z; Lan Y BMC Vet Res 2020[Aug]; 16 (1): 272 PMID32758221show ga BACKGROUND: Coronaviruses are notorious pathogens that cause diarrheic and respiratory diseases in humans and animals. Although the epidemiology and pathogenicity of coronaviruses have gained substantial attention, little is known about bovine coronavirus in cattle, which possesses a close relationship with human coronavirus. Bovine torovirus (BToV) is a newly identified relevant pathogen associated with cattle diarrhoea and respiratory diseases, and its epidemiology in the Chinese cattle industry remains unknown. RESULTS: In this study, a total of 461 diarrhoeic faecal samples were collected from 38 different farms in three intensive cattle farming regions and analysed. Our results demonstrated that BToV is present in China, with a low prevalence rate of 1.74% (8/461). The full-length spike genes were further cloned from eight clinical samples (five farms in Henan Province). Phylogenetic analysis showed that two different subclades of BToV strains are circulating in China. Meanwhile, the three BToV strains identified from dairy calves, 18,307, 2YY and 5YY, all contained the amino acid variants R614Q, I801T, N841S and Q885E. CONCLUSIONS: This is the first report to confirm the presence of BToV in beef and dairy calves in China with diarrhea, which extend our understanding of the epidemiology of BToVs worldwide. |Animals[MESH] |Cattle[MESH] |Cattle Diseases/epidemiology/*virology[MESH] |China/epidemiology[MESH] |Diarrhea/epidemiology/veterinary/virology[MESH] |Feces/virology[MESH] |Phylogeny[MESH] |RNA, Viral[MESH] |Sequence Analysis, DNA[MESH] |Torovirus Infections/epidemiology/*veterinary/virology[MESH] |Torovirus/genetics/*isolation & purification[MESH]First report and genetic characterization of bovine torovirus in diarr(epmc).pdf DeepDyve Pubget Overpricing