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10.1161/CIRCEP.120.008740

http://scihub22266oqcxt.onion/10.1161/CIRCEP.120.008740
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32755466!7578059!32755466
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suck abstract from ncbi


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pmid32755466      Circ+Arrhythm+Electrophysiol 2020 ; 13 (10): e008740
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  • Human Cardiac Mesenchymal Stem Cells Remodel in Disease and Can Regulate Arrhythmia Substrates #MMPMID32755466
  • Sattayaprasert P; Vasireddi SK; Bektik E; Jeon O; Hajjiri M; Mackall JA; Moravec CS; Alsberg E; Fu J; Laurita KR
  • Circ Arrhythm Electrophysiol 2020[Oct]; 13 (10): e008740 PMID32755466show ga
  • BACKGROUND: The mesenchymal stem cell (MSC), known to remodel in disease and have an extensive secretome, has recently been isolated from the human heart. However, the effects of normal and diseased cardiac MSCs on myocyte electrophysiology remain unclear. We hypothesize that in disease the inflammatory secretome of cardiac human MSCs (hMSCs) remodels and can regulate arrhythmia substrates. METHODS: hMSCs were isolated from patients with or without heart failure from tissue attached to extracted device leads and from samples taken from explanted/donor hearts. Failing hMSCs or nonfailing hMSCs were cocultured with normal human cardiac myocytes derived from induced pluripotent stem cells. Using fluorescent indicators, action potential duration, Ca2+ alternans, and spontaneous calcium release (SCR) incidence were determined. RESULTS: Failing and nonfailing hMSCs from both sources exhibited similar trilineage differentiation potential and cell surface marker expression as bone marrow hMSCs. Compared with nonfailing hMSCs, failing hMSCs prolonged action potential duration by 24% (P<0.001, n=15), increased Ca2+ alternans by 300% (P<0.001, n=18), and promoted spontaneous calcium release activity (n=14, P<0.013) in human cardiac myocytes derived from induced pluripotent stem cells. Failing hMSCs exhibited increased secretion of inflammatory cytokines IL (interleukin)-1beta (98%, P<0.0001) and IL-6 (460%, P<0.02) compared with nonfailing hMSCs. IL-1beta or IL-6 in the absence of hMSCs prolonged action potential duration but only IL-6 increased Ca2+ alternans and promoted spontaneous calcium release activity in human cardiac myocytes derived from induced pluripotent stem cells, replicating the effects of failing hMSCs. In contrast, nonfailing hMSCs prevented Ca2+ alternans in human cardiac myocytes derived from induced pluripotent stem cells during oxidative stress. Finally, nonfailing hMSCs exhibited >25x higher secretion of IGF (insulin-like growth factor)-1 compared with failing hMSCs. Importantly, IGF-1 supplementation or anti-IL-6 treatment rescued the arrhythmia substrates induced by failing hMSCs. CONCLUSIONS: We identified device leads as a novel source of cardiac hMSCs. Our findings show that cardiac hMSCs can regulate arrhythmia substrates by remodeling their secretome in disease. Importantly, therapy inhibiting (anti-IL-6) or mimicking (IGF-1) the cardiac hMSC secretome can rescue arrhythmia substrates.
  • |*Action Potentials[MESH]
  • |*Calcium Signaling[MESH]
  • |*Paracrine Communication[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Arrhythmias, Cardiac/*metabolism/pathology/physiopathology[MESH]
  • |Case-Control Studies[MESH]
  • |Cell Lineage[MESH]
  • |Cells, Cultured[MESH]
  • |Coculture Techniques[MESH]
  • |Female[MESH]
  • |Heart Failure/*metabolism/pathology/physiopathology[MESH]
  • |Humans[MESH]
  • |Induced Pluripotent Stem Cells/*metabolism/pathology[MESH]
  • |Inflammation Mediators/*metabolism[MESH]
  • |Interleukin-1beta/metabolism[MESH]
  • |Interleukin-6/metabolism[MESH]
  • |Kinetics[MESH]
  • |Male[MESH]
  • |Mesenchymal Stem Cells/*metabolism/pathology[MESH]
  • |Middle Aged[MESH]
  • |Myocytes, Cardiac/*metabolism/pathology[MESH]


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