Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1002/jcla.23507 http://scihub22266oqcxt.onion/10.1002/jcla.23507 32754967!7435359!32754967     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Lab+Anal 2020 ; 34 (10): e23507 Nephropedia Template TP {{tp|p=32754967|t=2020. Laboratory verification of an RT-PCR assay for SARS-CoV-2 |pdf=|usr=}}{{32754967}} gab.com Text Laboratory verification of an RT-PCR assay for SARS-CoV-2 JO: J Clin Lab Anal http://www.kidney.de/pget.php?&tw=1&pmid=32754967 #FOAvip BACKGROUND: Reverse transcription-polymerase chain reaction (RT-PCR) is an extremely common clinical method for detecting pathogens, particularly for emerging infectious diseases such as the new coronavirus disease (COVID-19). Currently, detection of the RNA from the novel coronavirus SARS-CoV-2 is the gold standard for establishing a COVID-19 diagnosis. This study evaluates the characteristic performance of the analytical system in a clinical laboratory. METHODS: A commercial SARS-CoV-2 RNA RT-PCR Kit used in a clinical laboratory is assessed based on ISO 15189 verification requirements. A multiple real-time RT-PCR assay for the RdRP, N, and E genes in SARS-CoV-2 is verified. RESULTS: The analytical system exhibits good analytical sensitivity (1000 copies/mL) and specificity (100%); however, the values of 86.7% and 100% for analytical accuracy deserved attention, compared with two other types of methods. Overall, the kit is potentially useful for SARS-CoV-2 diagnostic testing and meets the verification requirements. CONCLUSION: Compliance with international standards, such as ISO 15189, is valuable for clinical laboratories and for improving laboratory medicine quality and safety. Normalization is essential for obtaining reliable results from the SARS-CoV-2 RNA RT-PCR assay. This study aims to develop an improved SARS-CoV-2 verification framework compared with traditional molecular diagnostic methods, given the urgency of implementing new assays in clinical laboratories. Twit Text FOAVip Laboratory verification of an RT-PCR assay for SARS-CoV-2 ????J Clin Lab Anal http://www.kidney.de/pget.php?&tw=1&pmid=32754967 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32754967 #FOAvip English Wikipedia <ref>{{Cite pmid|32754967}}</ref> Laboratory verification of an RT-PCR assay for SARS-CoV-2 #MMPMID32754967 Wu Y; Xu W; Zhu Z; Xia X J Clin Lab Anal 2020[Oct]; 34 (10): e23507 PMID32754967show ga BACKGROUND: Reverse transcription-polymerase chain reaction (RT-PCR) is an extremely common clinical method for detecting pathogens, particularly for emerging infectious diseases such as the new coronavirus disease (COVID-19). Currently, detection of the RNA from the novel coronavirus SARS-CoV-2 is the gold standard for establishing a COVID-19 diagnosis. This study evaluates the characteristic performance of the analytical system in a clinical laboratory. METHODS: A commercial SARS-CoV-2 RNA RT-PCR Kit used in a clinical laboratory is assessed based on ISO 15189 verification requirements. A multiple real-time RT-PCR assay for the RdRP, N, and E genes in SARS-CoV-2 is verified. RESULTS: The analytical system exhibits good analytical sensitivity (1000 copies/mL) and specificity (100%); however, the values of 86.7% and 100% for analytical accuracy deserved attention, compared with two other types of methods. Overall, the kit is potentially useful for SARS-CoV-2 diagnostic testing and meets the verification requirements. CONCLUSION: Compliance with international standards, such as ISO 15189, is valuable for clinical laboratories and for improving laboratory medicine quality and safety. Normalization is essential for obtaining reliable results from the SARS-CoV-2 RNA RT-PCR assay. This study aims to develop an improved SARS-CoV-2 verification framework compared with traditional molecular diagnostic methods, given the urgency of implementing new assays in clinical laboratories. |*Clinical Laboratory Techniques/methods/standards[MESH] |*Molecular Diagnostic Techniques/methods/standards[MESH] |*Reverse Transcriptase Polymerase Chain Reaction/methods/standards[MESH] |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |COVID-19 Vaccines[MESH] |Coronavirus Infections/*diagnosis[MESH] |Humans[MESH] |Limit of Detection[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |Quality Control[MESH] |RNA, Viral/analysis[MESH] |Reproducibility of Results[MESH]Laboratory verification of an RT-PCR assay for SARS-CoV-2 (epmc).pdf DeepDyve Pubget Overpricing