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10.1038/s41594-020-0483-8 http://scihub22266oqcxt.onion/10.1038/s41594-020-0483-8 32753755!7541350!32753755     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Struct+Mol+Biol 2020 ; 27 (10): 942-949 Nephropedia Template TP {{tp|p=32753755|t=2020. Structure-guided covalent stabilization of coronavirus spike glycoprotein trimers in the closed conformation |pdf=|usr=}}{{32753755}} gab.com Text Structure-guided covalent stabilization of coronavirus spike glycoprotein trimers in the closed conformation JO: Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=32753755 #FOAvip SARS-CoV-2 is the causative agent of the COVID-19 pandemic, with 10 million infections and more than 500,000 fatalities by June 2020. To initiate infection, the SARS-CoV-2 spike (S) glycoprotein promotes attachment to the host cell surface and fusion of the viral and host membranes. Prefusion SARS-CoV-2 S is the main target of neutralizing antibodies and the focus of vaccine design. However, its limited stability and conformational dynamics are limiting factors for developing countermeasures against this virus. We report here the design of a construct corresponding to the prefusion SARS-CoV-2 S ectodomain trimer, covalently stabilized by a disulfide bond in the closed conformation. Structural and antigenicity analyses show we successfully shut S in the closed state without otherwise altering its architecture. We demonstrate that this strategy is applicable to other beta-coronaviruses, such as SARS-CoV and MERS-CoV, and might become an important tool for structural biology, serology, vaccine design and immunology studies. Twit Text FOAVip Structure-guided covalent stabilization of coronavirus spike glycoprotein trimers in the closed conformation ????Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=32753755 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32753755 #FOAvip English Wikipedia <ref>{{Cite pmid|32753755}}</ref> Structure-guided covalent stabilization of coronavirus spike glycoprotein trimers in the closed conformation #MMPMID32753755 McCallum M; Walls AC; Bowen JE; Corti D; Veesler D Nat Struct Mol Biol 2020[Oct]; 27 (10): 942-949 PMID32753755show ga SARS-CoV-2 is the causative agent of the COVID-19 pandemic, with 10 million infections and more than 500,000 fatalities by June 2020. To initiate infection, the SARS-CoV-2 spike (S) glycoprotein promotes attachment to the host cell surface and fusion of the viral and host membranes. Prefusion SARS-CoV-2 S is the main target of neutralizing antibodies and the focus of vaccine design. However, its limited stability and conformational dynamics are limiting factors for developing countermeasures against this virus. We report here the design of a construct corresponding to the prefusion SARS-CoV-2 S ectodomain trimer, covalently stabilized by a disulfide bond in the closed conformation. Structural and antigenicity analyses show we successfully shut S in the closed state without otherwise altering its architecture. We demonstrate that this strategy is applicable to other beta-coronaviruses, such as SARS-CoV and MERS-CoV, and might become an important tool for structural biology, serology, vaccine design and immunology studies. |Antibodies, Monoclonal/metabolism[MESH] |Antibodies, Neutralizing/metabolism[MESH] |Betacoronavirus/*chemistry/genetics/*immunology/metabolism[MESH] |Cryoelectron Microscopy[MESH] |Disulfides/chemistry[MESH] |Electrophoresis, Polyacrylamide Gel[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Humans[MESH] |Models, Molecular[MESH] |Mutation[MESH] |Protein Conformation[MESH] |Protein Domains[MESH] |Protein Engineering[MESH] |Protein Multimerization[MESH] |Protein Stability[MESH] |SARS-CoV-2[MESH]Structure-guided covalent stabilization of coronavirus spike glycoprot(epmc).pdf DeepDyve Pubget Overpricing