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10.3390/s20154289 http://scihub22266oqcxt.onion/10.3390/s20154289 32752043!7435936!32752043     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sensors+(Basel) 2020 ; 20 (15): ä Nephropedia Template TP {{tp|p=32752043|t=2020. Point-of-Care Diagnostics of COVID-19: From Current Work to Future Perspectives |pdf=|usr=}}{{32752043}} gab.com Text Point-of-Care Diagnostics of COVID-19: From Current Work to Future Perspectives JO: Sensors (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=32752043 #FOAvip Coronaviruses have received global concern since 2003, when an outbreak caused by SARS-CoV emerged in China. Later on, in 2012, the Middle-East respiratory syndrome spread in Saudi Arabia, caused by MERS-CoV. Currently, the global crisis is caused by the pandemic SARS-CoV-2, which belongs to the same lineage of SARS-CoV. In response to the urgent need of diagnostic tools, several lab-based and biosensing techniques have been proposed so far. Five main areas have been individuated and discussed in terms of their strengths and weaknesses. The cell-culture detection and the microneutralization tests are still considered highly reliable methods. The genetic screening, featuring the well-established Real-time polymerase chain reaction (RT-PCR), represents the gold standard for virus detection in nasopharyngeal swabs. On the other side, immunoassays were developed, either by screening/antigen recognition of IgM/IgG or by detecting the whole virus, in blood and sera. Next, proteomic mass-spectrometry (MS)-based methodologies have also been proposed for the analysis of swab samples. Finally, virus-biosensing devices were efficiently designed. Both electrochemical immunosensors and eye-based technologies have been described, showing detection times lower than 10 min after swab introduction. Alternative to swab-based techniques, lateral flow point-of-care immunoassays are already commercially available for the analysis of blood samples. Such biosensing devices hold the advantage of being portable for on-site testing in hospitals, airports, and hotspots, virtually without any sample treatment or complicated lab precautions. Twit Text FOAVip Point-of-Care Diagnostics of COVID-19: From Current Work to Future Perspectives ????Sensors (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=32752043 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32752043 #FOAvip English Wikipedia <ref>{{Cite pmid|32752043}}</ref> Point-of-Care Diagnostics of COVID-19: From Current Work to Future Perspectives #MMPMID32752043 Hussein HA; Hassan RYA; Chino M; Febbraio F Sensors (Basel) 2020[Jul]; 20 (15): ä PMID32752043show ga Coronaviruses have received global concern since 2003, when an outbreak caused by SARS-CoV emerged in China. Later on, in 2012, the Middle-East respiratory syndrome spread in Saudi Arabia, caused by MERS-CoV. Currently, the global crisis is caused by the pandemic SARS-CoV-2, which belongs to the same lineage of SARS-CoV. In response to the urgent need of diagnostic tools, several lab-based and biosensing techniques have been proposed so far. Five main areas have been individuated and discussed in terms of their strengths and weaknesses. The cell-culture detection and the microneutralization tests are still considered highly reliable methods. The genetic screening, featuring the well-established Real-time polymerase chain reaction (RT-PCR), represents the gold standard for virus detection in nasopharyngeal swabs. On the other side, immunoassays were developed, either by screening/antigen recognition of IgM/IgG or by detecting the whole virus, in blood and sera. Next, proteomic mass-spectrometry (MS)-based methodologies have also been proposed for the analysis of swab samples. Finally, virus-biosensing devices were efficiently designed. Both electrochemical immunosensors and eye-based technologies have been described, showing detection times lower than 10 min after swab introduction. Alternative to swab-based techniques, lateral flow point-of-care immunoassays are already commercially available for the analysis of blood samples. Such biosensing devices hold the advantage of being portable for on-site testing in hospitals, airports, and hotspots, virtually without any sample treatment or complicated lab precautions. |*Point-of-Care Systems[MESH] |Antibodies, Viral/blood[MESH] |Betacoronavirus/genetics/isolation & purification/metabolism[MESH] |Biosensing Techniques/methods[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis/virology[MESH] |Humans[MESH] |Immunoassay/methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/virology[MESH] |Proteomics/methods[MESH] |RNA, Viral/analysis/metabolism[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH]Point-of-Care Diagnostics of COVID-19: From Current Work to Future Per(epmc).pdf DeepDyve Pubget Overpricing