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10.1016/j.jcv.2020.104567 http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104567 32750665!7392849!32750665     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2020 ; 130 (ä): 104567 Nephropedia Template TP {{tp|p=32750665|t=2020. Challenges in use of saliva for detection of SARS CoV-2 RNA in symptomatic outpatients |pdf=|usr=}}{{32750665}} gab.com Text Challenges in use of saliva for detection of SARS CoV-2 RNA in symptomatic outpatients JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32750665 #FOAvip BACKGROUND: A major expansion in SARS CoV-2 testing is urgently needed. Saliva is an attractive option as an alternative for nasopharyngeal swabs (NPS), since saliva can be self-collected, is non-invasive, and sample quality is not dependent on the expertise of the collector. OBJECTIVE: To compare SARS CoV-2 positivity on paired NPS and saliva samples. STUDY DESIGN: NPS and paired saliva samples were prospectively collected from symptomatic outpatients suspected of having COVID-19 and were tested by real-time RT-PCR. RESULTS: In total, 35/124 (26.6 %) samples were RT-PCR positive, with 33/35 positive by NPS (sensitivity?=?94.3 % (95 % CI 81.4%-99.0%)) and 30/35 by pure saliva (sensitivity?=?85.7 % (95 % CI 70.6%-93.7%)), for an overall agreement of 117/124 (94.4 %). The median cycle threshold value was significantly lower for NPS than for saliva (p?=?0.0331). A third or more of pure saliva samples from symptomatic patients were thick, stringy, and difficult to pipet. CONCLUSIONS: Real-time RT-PCR of pure saliva had an overall sensitivity for SARS CoV-2 RNA detection of 85.7 % when compared to simultaneously collected NPS. Our study highlighted the need to optimize collection and processing before saliva can be used for high volume testing. Twit Text FOAVip Challenges in use of saliva for detection of SARS CoV-2 RNA in symptomatic outpatients ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32750665 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32750665 #FOAvip English Wikipedia <ref>{{Cite pmid|32750665}}</ref> Challenges in use of saliva for detection of SARS CoV-2 RNA in symptomatic outpatients #MMPMID32750665 Landry ML; Criscuolo J; Peaper DR J Clin Virol 2020[Sep]; 130 (ä): 104567 PMID32750665show ga BACKGROUND: A major expansion in SARS CoV-2 testing is urgently needed. Saliva is an attractive option as an alternative for nasopharyngeal swabs (NPS), since saliva can be self-collected, is non-invasive, and sample quality is not dependent on the expertise of the collector. OBJECTIVE: To compare SARS CoV-2 positivity on paired NPS and saliva samples. STUDY DESIGN: NPS and paired saliva samples were prospectively collected from symptomatic outpatients suspected of having COVID-19 and were tested by real-time RT-PCR. RESULTS: In total, 35/124 (26.6 %) samples were RT-PCR positive, with 33/35 positive by NPS (sensitivity?=?94.3 % (95 % CI 81.4%-99.0%)) and 30/35 by pure saliva (sensitivity?=?85.7 % (95 % CI 70.6%-93.7%)), for an overall agreement of 117/124 (94.4 %). The median cycle threshold value was significantly lower for NPS than for saliva (p?=?0.0331). A third or more of pure saliva samples from symptomatic patients were thick, stringy, and difficult to pipet. CONCLUSIONS: Real-time RT-PCR of pure saliva had an overall sensitivity for SARS CoV-2 RNA detection of 85.7 % when compared to simultaneously collected NPS. Our study highlighted the need to optimize collection and processing before saliva can be used for high volume testing. |Betacoronavirus[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |COVID-19 Vaccines[MESH] |Clinical Laboratory Techniques[MESH] |Coronavirus Infections/*diagnosis[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/*standards[MESH] |Nasopharynx/virology[MESH] |Outpatients[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |Prospective Studies[MESH] |RNA, Viral/*analysis[MESH] |Real-Time Polymerase Chain Reaction[MESH] |SARS-CoV-2[MESH] |Saliva/*virology[MESH]Challenges in use of saliva for detection of SARS CoV-2 RNA in symptom(epmc).pdf DeepDyve Pubget Overpricing