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10.4049/jimmunol.1901489 http://scihub22266oqcxt.onion/10.4049/jimmunol.1901489 32747502!9202338!32747502     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Immunol 2020 ; 205 (6): 1564-1579 Nephropedia Template TP {{tp|p=32747502|t=2020. Middle East Respiratory Syndrome Coronavirus ORF8b Accessory Protein Suppresses Type I IFN Expression by Impeding HSP70-Dependent Activation of IRF3 Kinase IKKepsilon |pdf=|usr=}}{{32747502}} gab.com Text Middle East Respiratory Syndrome Coronavirus ORF8b Accessory Protein Suppresses Type I IFN Expression by Impeding HSP70-Dependent Activation of IRF3 Kinase IKKepsilon JO: J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=32747502 #FOAvip Middle East respiratory syndrome coronavirus (MERS-CoV) is a highly pathogenic human coronavirus causing severe disease and mortality. MERS-CoV infection failed to elicit robust IFN response, suggesting that the virus might have evolved strategies to evade host innate immune surveillance. In this study, we identified and characterized type I IFN antagonism of MERS-CoV open reading frame (ORF) 8b accessory protein. ORF8b was abundantly expressed in MERS-CoV-infected Huh-7 cells. When ectopically expressed, ORF8b inhibited IRF3-mediated IFN-beta expression induced by Sendai virus and poly(I:C). ORF8b was found to act at a step upstream of IRF3 to impede the interaction between IRF3 kinase IKKepsilon and chaperone protein HSP70, which is required for the activation of IKKepsilon and IRF3. An infection study using recombinant wild-type and ORF8b-deficient MERS-CoV further confirmed the suppressive role of ORF8b in type I IFN induction and its disruption of the colocalization of HSP70 with IKKepsilon. Ectopic expression of HSP70 relieved suppression of IFN-beta expression by ORF8b in an IKKepsilon-dependent manner. Enhancement of IFN-beta induction in cells infected with ORF8b-deficient virus was erased when HSP70 was depleted. Taken together, HSP70 chaperone is important for IKKepsilon activation, and MERS-CoV ORF8b suppresses type I IFN expression by competing with IKKepsilon for interaction with HSP70. Twit Text FOAVip Middle East Respiratory Syndrome Coronavirus ORF8b Accessory Protein Suppresses Type I IFN Expression by Impeding HSP70-Dependent Activation of IRF3 Kinase IKKepsilon ????J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=32747502 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32747502 #FOAvip English Wikipedia <ref>{{Cite pmid|32747502}}</ref> Middle East Respiratory Syndrome Coronavirus ORF8b Accessory Protein Suppresses Type I IFN Expression by Impeding HSP70-Dependent Activation of IRF3 Kinase IKKepsilon #MMPMID32747502 Wong LR; Ye ZW; Lui PY; Zheng X; Yuan S; Zhu L; Fung SY; Yuen KS; Siu KL; Yeung ML; Cai Z; Woo PC; Yuen KY; Chan CP; Jin DY J Immunol 2020[Sep]; 205 (6): 1564-1579 PMID32747502show ga Middle East respiratory syndrome coronavirus (MERS-CoV) is a highly pathogenic human coronavirus causing severe disease and mortality. MERS-CoV infection failed to elicit robust IFN response, suggesting that the virus might have evolved strategies to evade host innate immune surveillance. In this study, we identified and characterized type I IFN antagonism of MERS-CoV open reading frame (ORF) 8b accessory protein. ORF8b was abundantly expressed in MERS-CoV-infected Huh-7 cells. When ectopically expressed, ORF8b inhibited IRF3-mediated IFN-beta expression induced by Sendai virus and poly(I:C). ORF8b was found to act at a step upstream of IRF3 to impede the interaction between IRF3 kinase IKKepsilon and chaperone protein HSP70, which is required for the activation of IKKepsilon and IRF3. An infection study using recombinant wild-type and ORF8b-deficient MERS-CoV further confirmed the suppressive role of ORF8b in type I IFN induction and its disruption of the colocalization of HSP70 with IKKepsilon. Ectopic expression of HSP70 relieved suppression of IFN-beta expression by ORF8b in an IKKepsilon-dependent manner. Enhancement of IFN-beta induction in cells infected with ORF8b-deficient virus was erased when HSP70 was depleted. Taken together, HSP70 chaperone is important for IKKepsilon activation, and MERS-CoV ORF8b suppresses type I IFN expression by competing with IKKepsilon for interaction with HSP70. |Betacoronavirus[MESH] |COVID-19[MESH] |Cell Line[MESH] |Coronavirus Infections[MESH] |Enzyme Activation/*immunology[MESH] |HSP70 Heat-Shock Proteins/immunology/metabolism[MESH] |Humans[MESH] |I-kappa B Kinase/*immunology/metabolism[MESH] |Interferon Type I/*immunology/metabolism[MESH] |Middle East Respiratory Syndrome Coronavirus/*immunology/metabolism[MESH] |Pandemics[MESH] |Pneumonia, Viral[MESH] |SARS-CoV-2[MESH]Middle East Respiratory Syndrome Coronavirus ORF8b Accessory Protein S(epmc).pdf DeepDyve Pubget Overpricing