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10.1016/j.ijid.2020.07.058 http://scihub22266oqcxt.onion/10.1016/j.ijid.2020.07.058 32745627!7395224!32745627     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Infect+Dis 2020 ; 99 (ä): 190-192 Nephropedia Template TP {{tp|p=32745627|t=2020. Sensitivity of different RT-qPCR solutions for SARS-CoV-2 detection |pdf=|usr=}}{{32745627}} gab.com Text Sensitivity of different RT-qPCR solutions for SARS-CoV-2 detection JO: Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32745627 #FOAvip OBJECTIVES: The ongoing COVID-19 pandemic continues to impose demands on diagnostic screening. In anticipation that the recurrence of outbreaks and the measures for lifting the lockdown worldwide may cause supply chain issues over the coming months, this study assessed the sensitivity of a number of one-step retrotranscription and quantitative polymerase chain reaction (RT-qPCR) solutions to detect SARS-CoV-2. METHODS: Six different RT-qPCR alternatives were evaluated for SARS-CoV-2/COVID-19 diagnosis based on standard RNA extractions. The one with best sensitivity was also assessed with direct nasopharyngeal swab viral transmission medium (VTM) heating; thus overcoming the RNA extraction step. RESULTS: A wide variability in the sensitivity of RT-qPCR solutions was found that was associated with a range of false negatives from 2% (0.3-7.9%) to 39.8% (30.2-50.2%). Direct preheating of VTM combined with the best solution provided a sensitivity of 72.5% (62.5-81.0%), in the range of some of the solutions based on standard RNA extractions. CONCLUSIONS: Sensitivity limitations of currently used RT-qPCR solutions were found. These results will help to calibrate the impact of false negative diagnoses of COVID-19, and to detect and control new SARS-CoV-2 outbreaks and community transmissions. Twit Text FOAVip Sensitivity of different RT-qPCR solutions for SARS-CoV-2 detection ????Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32745627 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32745627 #FOAvip English Wikipedia <ref>{{Cite pmid|32745627}}</ref> Sensitivity of different RT-qPCR solutions for SARS-CoV-2 detection #MMPMID32745627 Alcoba-Florez J; Gil-Campesino H; Artola DG; Gonzalez-Montelongo R; Valenzuela-Fernandez A; Ciuffreda L; Flores C Int J Infect Dis 2020[Oct]; 99 (ä): 190-192 PMID32745627show ga OBJECTIVES: The ongoing COVID-19 pandemic continues to impose demands on diagnostic screening. In anticipation that the recurrence of outbreaks and the measures for lifting the lockdown worldwide may cause supply chain issues over the coming months, this study assessed the sensitivity of a number of one-step retrotranscription and quantitative polymerase chain reaction (RT-qPCR) solutions to detect SARS-CoV-2. METHODS: Six different RT-qPCR alternatives were evaluated for SARS-CoV-2/COVID-19 diagnosis based on standard RNA extractions. The one with best sensitivity was also assessed with direct nasopharyngeal swab viral transmission medium (VTM) heating; thus overcoming the RNA extraction step. RESULTS: A wide variability in the sensitivity of RT-qPCR solutions was found that was associated with a range of false negatives from 2% (0.3-7.9%) to 39.8% (30.2-50.2%). Direct preheating of VTM combined with the best solution provided a sensitivity of 72.5% (62.5-81.0%), in the range of some of the solutions based on standard RNA extractions. CONCLUSIONS: Sensitivity limitations of currently used RT-qPCR solutions were found. These results will help to calibrate the impact of false negative diagnoses of COVID-19, and to detect and control new SARS-CoV-2 outbreaks and community transmissions. |Betacoronavirus/genetics/*isolation & purification[MESH] |COVID-19[MESH] |Coronavirus Envelope Proteins[MESH] |Coronavirus Infections/*diagnosis/*virology[MESH] |False Negative Reactions[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/*virology[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH]Sensitivity of different RT-qPCR solutions for SARS-CoV-2 detection (epmc).pdf DeepDyve Pubget Overpricing