Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.antiviral.2020.104882 http://scihub22266oqcxt.onion/10.1016/j.antiviral.2020.104882 32738255!7388004!32738255     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Antiviral+Res 2020 ; 181 (ä): 104882 Nephropedia Template TP {{tp|p=32738255|t=2020. An enzyme-based immunodetection assay to quantify SARS-CoV-2 infection |pdf=|usr=}}{{32738255}} gab.com Text An enzyme-based immunodetection assay to quantify SARS-CoV-2 infection JO: Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=32738255 #FOAvip SARS-CoV-2 is a novel pandemic coronavirus that caused a global health and economic crisis. The development of efficient drugs and vaccines against COVID-19 requires detailed knowledge about SARS-CoV-2 biology. Several techniques to detect SARS-CoV-2 infection have been established, mainly based on counting infected cells by staining plaques or foci, or by quantifying the viral genome by PCR. These methods are laborious, time-consuming and expensive and therefore not suitable for a high sample throughput or rapid diagnostics. We here report a novel enzyme-based immunodetection assay that directly quantifies the amount of de novo synthesized viral spike protein within fixed and permeabilized cells. This in-cell ELISA enables a rapid and quantitative detection of SARS-CoV-2 infection in microtiter format, regardless of the virus isolate or target cell culture. It follows the established method of performing ELISA assays and does not require expensive instrumentation. Utilization of the in-cell ELISA allows to e.g. determine TCID(50) of virus stocks, antiviral efficiencies (IC(50) values) of drugs or neutralizing activity of sera. Thus, the in-cell spike ELISA represents a promising alternative to study SARS-CoV-2 infection and inhibition and may facilitate future research. Twit Text FOAVip An enzyme-based immunodetection assay to quantify SARS-CoV-2 infection ????Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=32738255 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32738255 #FOAvip English Wikipedia <ref>{{Cite pmid|32738255}}</ref> An enzyme-based immunodetection assay to quantify SARS-CoV-2 infection #MMPMID32738255 Conzelmann C; Gilg A; Gross R; Schutz D; Preising N; Standker L; Jahrsdorfer B; Schrezenmeier H; Sparrer KMJ; Stamminger T; Stenger S; Munch J; Muller JA Antiviral Res 2020[Sep]; 181 (ä): 104882 PMID32738255show ga SARS-CoV-2 is a novel pandemic coronavirus that caused a global health and economic crisis. The development of efficient drugs and vaccines against COVID-19 requires detailed knowledge about SARS-CoV-2 biology. Several techniques to detect SARS-CoV-2 infection have been established, mainly based on counting infected cells by staining plaques or foci, or by quantifying the viral genome by PCR. These methods are laborious, time-consuming and expensive and therefore not suitable for a high sample throughput or rapid diagnostics. We here report a novel enzyme-based immunodetection assay that directly quantifies the amount of de novo synthesized viral spike protein within fixed and permeabilized cells. This in-cell ELISA enables a rapid and quantitative detection of SARS-CoV-2 infection in microtiter format, regardless of the virus isolate or target cell culture. It follows the established method of performing ELISA assays and does not require expensive instrumentation. Utilization of the in-cell ELISA allows to e.g. determine TCID(50) of virus stocks, antiviral efficiencies (IC(50) values) of drugs or neutralizing activity of sera. Thus, the in-cell spike ELISA represents a promising alternative to study SARS-CoV-2 infection and inhibition and may facilitate future research. |Animals[MESH] |Antibodies, Viral/*immunology[MESH] |Betacoronavirus/*immunology/isolation & purification[MESH] |COVID-19[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/diagnosis/*virology[MESH] |Enzyme-Linked Immunosorbent Assay/*methods[MESH] |Humans[MESH] |Pandemics[MESH] |Pneumonia, Viral/diagnosis/*virology[MESH] |SARS-CoV-2[MESH] |Severe Acute Respiratory Syndrome/diagnosis/*virology[MESH] |Spike Glycoprotein, Coronavirus/genetics/*immunology[MESH] |Vero Cells[MESH]An enzyme-based immunodetection assay to quantify SARS-CoV-2 infection(epmc).pdf DeepDyve Pubget Overpricing