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10.1093/infdis/jiaa487

http://scihub22266oqcxt.onion/10.1093/infdis/jiaa487
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32738137!7454747!32738137
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suck abstract from ncbi


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pmid32738137      J+Infect+Dis 2020 ; 222 (10): 1612-1619
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  • Prospective Study Comparing Deep Throat Saliva With Other Respiratory Tract Specimens in the Diagnosis of Novel Coronavirus Disease 2019 #MMPMID32738137
  • Lai CKC; Chen Z; Lui G; Ling L; Li T; Wong MCS; Ng RWY; Tso EYK; Ho T; Fung KSC; Ng ST; Wong BKC; Boon SS; Hui DSC; Chan PKS
  • J Infect Dis 2020[Oct]; 222 (10): 1612-1619 PMID32738137show ga
  • BACKGROUND: Self-collected specimens have been advocated to avoid infectious exposure to healthcare workers. Self-induced sputum in those with a productive cough and saliva in those without a productive cough have been proposed, but sensitivity remains uncertain. METHODS: We performed a prospective study in 2 regional hospitals in Hong Kong. RESULTS: We prospectively examined 563 serial samples collected during the virus shedding periods of 50 patients: 150 deep throat saliva (DTS), 309 pooled-nasopharyngeal (NP) and throat swabs, and 104 sputum. Deep throat saliva had the lowest overall reverse-transcriptase polymerase chain reaction (RT-PCR)-positive rate (68.7% vs 89.4% [sputum] and 80.9% [pooled NP and throat swabs]) and the lowest viral ribonucleic acid (RNA) concentration (mean log copy/mL 3.54 vs 5.03 [sputum] and 4.63 [pooled NP and throat swabs]). Analyses with respect to time from symptom onset and severity also revealed similar results. Virus yields of DTS correlated with that of sputum (Pearson correlation index 0.76; 95% confidence interval, 0.62-0.86). We estimated that the overall false-negative rate of DTS could be as high as 31.3% and increased 2.7 times among patients without sputum. CONCLUSIONS: Deep throat saliva produced the lowest viral RNA concentration and RT-PCR-positive rate compared with conventional respiratory specimens in all phases of illness. Self-collected sputum should be the choice for patients with sputum.
  • |*Clinical Laboratory Techniques/methods[MESH]
  • |Adolescent[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Betacoronavirus/*genetics[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |COVID-19 Vaccines[MESH]
  • |Coronavirus Infections/*diagnosis/*epidemiology/virology[MESH]
  • |Female[MESH]
  • |Hong Kong/epidemiology[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Nasopharynx/*virology[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/*epidemiology/virology[MESH]
  • |Prospective Studies[MESH]
  • |RNA, Viral/genetics[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2[MESH]
  • |Saliva/*virology[MESH]
  • |Specimen Handling/methods[MESH]
  • |Sputum/*virology[MESH]


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