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10.1016/j.chom.2020.06.021 http://scihub22266oqcxt.onion/10.1016/j.chom.2020.06.021 32735849!7332453!32735849     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Host+Microbe 2020 ; 28 (3): 475-485.e5 Nephropedia Template TP {{tp|p=32735849|t=2020. Neutralizing Antibody and Soluble ACE2 Inhibition of a Replication-Competent VSV-SARS-CoV-2 and a Clinical Isolate of SARS-CoV-2 |pdf=|usr=}}{{32735849}} gab.com Text Neutralizing Antibody and Soluble ACE2 Inhibition of a Replication-Competent VSV-SARS-CoV-2 and a Clinical Isolate of SARS-CoV-2 JO: Cell Host Microbe http://www.kidney.de/pget.php?&tw=1&pmid=32735849 #FOAvip Antibody-based interventions against SARS-CoV-2 could limit morbidity, mortality, and possibly transmission. An anticipated correlate of such countermeasures is the level of neutralizing antibodies against the SARS-CoV-2 spike protein, which engages with host ACE2 receptor for entry. Using an infectious molecular clone of vesicular stomatitis virus (VSV) expressing eGFP as a marker of infection, we replaced the glycoprotein gene (G) with the spike protein of SARS-CoV-2 (VSV-eGFP-SARS-CoV-2) and developed a high-throughput-imaging-based neutralization assay at biosafety level 2. We also developed a focus-reduction neutralization test with a clinical isolate of SARS-CoV-2 at biosafety level 3. Comparing the neutralizing activities of various antibodies and ACE2-Fc soluble decoy protein in both assays revealed a high degree of concordance. These assays will help define correlates of protection for antibody-based countermeasures and vaccines against SARS-CoV-2. Additionally, replication-competent VSV-eGFP-SARS-CoV-2 provides a tool for testing inhibitors of SARS-CoV-2 mediated entry under reduced biosafety containment. Twit Text FOAVip Neutralizing Antibody and Soluble ACE2 Inhibition of a Replication-Competent VSV-SARS-CoV-2 and a Clinical Isolate of SARS-CoV-2 ????Cell Host Microbe http://www.kidney.de/pget.php?&tw=1&pmid=32735849 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32735849 #FOAvip English Wikipedia <ref>{{Cite pmid|32735849}}</ref> Neutralizing Antibody and Soluble ACE2 Inhibition of a Replication-Competent VSV-SARS-CoV-2 and a Clinical Isolate of SARS-CoV-2 #MMPMID32735849 Case JB; Rothlauf PW; Chen RE; Liu Z; Zhao H; Kim AS; Bloyet LM; Zeng Q; Tahan S; Droit L; Ilagan MXG; Tartell MA; Amarasinghe G; Henderson JP; Miersch S; Ustav M; Sidhu S; Virgin HW; Wang D; Ding S; Corti D; Theel ES; Fremont DH; Diamond MS; Whelan SPJ Cell Host Microbe 2020[Sep]; 28 (3): 475-485.e5 PMID32735849show ga Antibody-based interventions against SARS-CoV-2 could limit morbidity, mortality, and possibly transmission. An anticipated correlate of such countermeasures is the level of neutralizing antibodies against the SARS-CoV-2 spike protein, which engages with host ACE2 receptor for entry. Using an infectious molecular clone of vesicular stomatitis virus (VSV) expressing eGFP as a marker of infection, we replaced the glycoprotein gene (G) with the spike protein of SARS-CoV-2 (VSV-eGFP-SARS-CoV-2) and developed a high-throughput-imaging-based neutralization assay at biosafety level 2. We also developed a focus-reduction neutralization test with a clinical isolate of SARS-CoV-2 at biosafety level 3. Comparing the neutralizing activities of various antibodies and ACE2-Fc soluble decoy protein in both assays revealed a high degree of concordance. These assays will help define correlates of protection for antibody-based countermeasures and vaccines against SARS-CoV-2. Additionally, replication-competent VSV-eGFP-SARS-CoV-2 provides a tool for testing inhibitors of SARS-CoV-2 mediated entry under reduced biosafety containment. |Angiotensin-Converting Enzyme 2[MESH] |Animals[MESH] |Antibodies, Neutralizing/*blood[MESH] |Antibodies, Viral/*blood[MESH] |Betacoronavirus/genetics/*immunology/physiology[MESH] |COVID-19[MESH] |COVID-19 Serotherapy[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/genetics/immunology/*therapy/virology[MESH] |Green Fluorescent Proteins/genetics[MESH] |Host Microbial Interactions/immunology[MESH] |Humans[MESH] |Immunization, Passive[MESH] |Neutralization Tests[MESH] |Pandemics[MESH] |Peptidyl-Dipeptidase A/*immunology[MESH] |Pneumonia, Viral/immunology/*therapy/virology[MESH] |SARS-CoV-2[MESH] |Spike Glycoprotein, Coronavirus/genetics/immunology[MESH] |Vero Cells[MESH] |Vesicular stomatitis Indiana virus/genetics/immunology[MESH] |Virus Internalization[MESH]Neutralizing Antibody and Soluble ACE2 Inhibition of a Replication-Com(epmc).pdf DeepDyve Pubget Overpricing