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10.3390/v12080812 http://scihub22266oqcxt.onion/10.3390/v12080812 32731335!7472224!32731335     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2020 ; 12 (8): ä Nephropedia Template TP {{tp|p=32731335|t=2020. Structural and Biological Basis of Alphacoronavirus nsp1 Associated with Host Proliferation and Immune Evasion |pdf=|usr=}}{{32731335}} gab.com Text Structural and Biological Basis of Alphacoronavirus nsp1 Associated with Host Proliferation and Immune Evasion JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32731335 #FOAvip Non-structural protein 1 (nsp1) is only characterized in alphacoronaviruses (alpha-CoVs) and betacoronaviruses (beta-CoVs). There have been extensive researches on how the beta-CoVs nsp1 regulates viral virulence by inhibiting host protein synthesis, but the regulatory mechanism of the alpha-CoVs nsp1 is still unclear. Here, we report the 2.1-A full-length crystal structure of nsp1 in emerging porcine SADS-CoV and the 1.8-A full-length crystal structure of nsp1 in the highly lethal cat FIPV. Although they belong to different subtypes of alpha-CoVs, these viruses all have a bucket-shaped fold composed of six beta-sheets, similar to the crystal structure of PEDV and TGEV nsp1. Comparing the above four structures, we found that the structure of alpha-CoVs nsp1 in the same subtype was more conserved. We then selected mammalian cells that were treated with SADS-CoV and FIPV nsp1 for RNA sequencing analysis and found that nsp1 had a specific inhibitory effect on interferon (IFN) and cell cycle genes. Using the Renilla luciferase (Rluc) assay and Western blotting, we confirmed that seven representative alpha-CoVs nsp1s could significantly inhibit the phosphorylation of STAT1-S727 and interfere with the effect of IFN-I. Moreover, the cell cycle experiment confirmed that alpha-CoVs nsp1 could encourage host cells to stay in the G0/G1 phase. Based on these findings, we not only greatly improved the crystal structure data on alpha-CoVs nsp1, but we also speculated that alpha-CoVs nsp1 regulated host proliferation and immune evasion-related biological functions by inhibiting the synthesis of host proteins, thus creating an environment conducive to the virus. Twit Text FOAVip Structural and Biological Basis of Alphacoronavirus nsp1 Associated with Host Proliferation and Immune Evasion ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32731335 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32731335 #FOAvip English Wikipedia <ref>{{Cite pmid|32731335}}</ref> Structural and Biological Basis of Alphacoronavirus nsp1 Associated with Host Proliferation and Immune Evasion #MMPMID32731335 Shen Z; Yang Y; Yang S; Zhang G; Xiao S; Fu ZF; Peng G Viruses 2020[Jul]; 12 (8): ä PMID32731335show ga Non-structural protein 1 (nsp1) is only characterized in alphacoronaviruses (alpha-CoVs) and betacoronaviruses (beta-CoVs). There have been extensive researches on how the beta-CoVs nsp1 regulates viral virulence by inhibiting host protein synthesis, but the regulatory mechanism of the alpha-CoVs nsp1 is still unclear. Here, we report the 2.1-A full-length crystal structure of nsp1 in emerging porcine SADS-CoV and the 1.8-A full-length crystal structure of nsp1 in the highly lethal cat FIPV. Although they belong to different subtypes of alpha-CoVs, these viruses all have a bucket-shaped fold composed of six beta-sheets, similar to the crystal structure of PEDV and TGEV nsp1. Comparing the above four structures, we found that the structure of alpha-CoVs nsp1 in the same subtype was more conserved. We then selected mammalian cells that were treated with SADS-CoV and FIPV nsp1 for RNA sequencing analysis and found that nsp1 had a specific inhibitory effect on interferon (IFN) and cell cycle genes. Using the Renilla luciferase (Rluc) assay and Western blotting, we confirmed that seven representative alpha-CoVs nsp1s could significantly inhibit the phosphorylation of STAT1-S727 and interfere with the effect of IFN-I. Moreover, the cell cycle experiment confirmed that alpha-CoVs nsp1 could encourage host cells to stay in the G0/G1 phase. Based on these findings, we not only greatly improved the crystal structure data on alpha-CoVs nsp1, but we also speculated that alpha-CoVs nsp1 regulated host proliferation and immune evasion-related biological functions by inhibiting the synthesis of host proteins, thus creating an environment conducive to the virus. |Alphacoronavirus/genetics/*immunology/*physiology[MESH] |Amino Acid Sequence[MESH] |Animals[MESH] |Cats[MESH] |Cell Line[MESH] |Crystallography, X-Ray[MESH] |G1 Phase Cell Cycle Checkpoints/physiology[MESH] |Gene Expression Regulation/genetics[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Immune Evasion/*immunology[MESH] |Interferon Type I/*antagonists & inhibitors[MESH] |Phosphorylation[MESH] |Protein Structure, Tertiary[MESH] |STAT1 Transcription Factor/metabolism[MESH] |Sequence Homology[MESH] |Swine[MESH] |Viral Nonstructural Proteins/genetics/*metabolism[MESH]Structural and Biological Basis of Alphacoronavirus nsp1 Associated wi(epmc).pdf DeepDyve Pubget Overpricing