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Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Virulence 2020 ; 11 (1): 964-967 Nephropedia Template TP
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Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed #MMPMID32726172
Mayer FJ; Ratzinger F; Schmidt RLJ; Greiner G; Landt O; Am Ende A; Corman VM; Perkmann-Nagele N; Watkins-Riedel T; Petermann D; Abadir K; Zweimuller-Mayer J; Strassl R
Virulence 2020[Dec]; 11 (1): 964-967 PMID32726172show ga
Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84-97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time.