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10.1080/21505594.2020.1798041

http://scihub22266oqcxt.onion/10.1080/21505594.2020.1798041
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32726172!7549918!32726172
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suck abstract from ncbi


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pmid32726172      Virulence 2020 ; 11 (1): 964-967
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  • Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed #MMPMID32726172
  • Mayer FJ; Ratzinger F; Schmidt RLJ; Greiner G; Landt O; Am Ende A; Corman VM; Perkmann-Nagele N; Watkins-Riedel T; Petermann D; Abadir K; Zweimuller-Mayer J; Strassl R
  • Virulence 2020[Dec]; 11 (1): 964-967 PMID32726172show ga
  • Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84-97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time.
  • |Betacoronavirus/genetics/*isolation & purification[MESH]
  • |High-Throughput Nucleotide Sequencing/instrumentation/*methods/standards[MESH]
  • |Humans[MESH]
  • |RNA, Viral/genetics/*isolation & purification[MESH]
  • |Real-Time Polymerase Chain Reaction/instrumentation/*methods/standards[MESH]
  • |Reproducibility of Results[MESH]
  • |SARS-CoV-2[MESH]
  • |Sensitivity and Specificity[MESH]


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