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Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Biochem+Biophys+Res+Commun 2020 ; 529 (2): 257-262 Nephropedia Template TP
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Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system #MMPMID32703420
Fujita R; Hino M; Ebihara T; Nagasato T; Masuda A; Lee JM; Fujii T; Mon H; Kakino K; Nagai R; Tanaka M; Tonooka Y; Moriyama T; Kusakabe T
Biochem Biophys Res Commun 2020[Aug]; 529 (2): 257-262 PMID32703420show ga
In the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines.