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suck abstract from ncbi


10.1016/j.clinbiochem.2020.07.002

http://scihub22266oqcxt.onion/10.1016/j.clinbiochem.2020.07.002
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32702365!7371578!32702365
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suck abstract from ncbi


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pmid32702365      Clin+Biochem 2020 ; 84 (ä): 87-92
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  • Comparison of serologic and molecular SARS-CoV 2 results in a large cohort in Southern Tuscany demonstrates a role for serologic testing to increase diagnostic sensitivity #MMPMID32702365
  • Pancrazzi A; Magliocca P; Lorubbio M; Vaggelli G; Galano A; Mafucci M; Duranti D; Cortesi M; Mazzeschi E; Fabbroni S; Viti G; Tartaglia Polcini A; Tripodo E; Sanchini P; Gervino S; Tacconi D; Dei S; Mazzierli M; D'Urso A; Ognibene A
  • Clin Biochem 2020[Oct]; 84 (ä): 87-92 PMID32702365show ga
  • BACKGROUND: Since February 2020, Italian hospitals registered COVID-19 (COronaVIrus Disease 19) cases more often than the rest of the Europe. During this epidemic, health authorities requested swab tests, while seeking new patient paths. METHODS: A dual laboratory approach was evaluated, consisting of patient care reports for viral RNA detection on swabs and rapid serological tests in 516 patients (192 symptomatic or paucisymptomatic and 324 asymptomatic). RESULTS: We found the molecular positive fraction equal to 12% (23/192) among symptomatic/paucisymptomatic (S/P) and 15.4% (50/324) in asymptomatic (As) sets. Among subsets, we observed serologically positive results, corresponding to 35% (8/23) for S/P and 38% (19/50) for As. Among molecular negative cases, we detected specific Immunoglobulin G or M (Ig G or Ig M) positivity in the S/P cohort equal to 6.6% (11/167) and 6% (15/246) in As cases. For indeterminate molecular results, we found S/P serological positivity equal to 100% (1/1) and 54% (13/24) in As patients. We found higher (p < 0.05) seropositivity in older patients (n = 8) among symptomatic and positives for viral RNA (n.23). CONCLUSIONS: It has been observed that a dual approach of serological and molecular tests detects a higher absolute number of disease cases in a pandemic context,which could improve monitoring and health surveillance efficacy. The age-related seropositivity frequency in this study, if confirmed, could enhance the validity of serological tests, especially in older patients.In these subjects, molecular positivity accompanied by serological positivity (distinct for M and G immunoglobulins) should help determine disease status and support decisions related to patient management.
  • |Aged[MESH]
  • |Antibodies, Viral/*blood[MESH]
  • |Betacoronavirus/*immunology/isolation & purification[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques/*methods/standards[MESH]
  • |Cohort Studies[MESH]
  • |Coronavirus Infections/blood/*diagnosis/epidemiology/virology[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Incidence[MESH]
  • |Italy/epidemiology[MESH]
  • |Male[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/blood/*diagnosis/epidemiology/virology[MESH]
  • |ROC Curve[MESH]
  • |SARS-CoV-2[MESH]


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