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10.1016/j.scitotenv.2020.140911 http://scihub22266oqcxt.onion/10.1016/j.scitotenv.2020.140911 32693284!7358170!32693284     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Total+Environ 2020 ; 744 (ä): 140911 Nephropedia Template TP {{tp|p=32693284|t=2020. Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers |pdf=|usr=}}{{32693284}} gab.com Text Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers JO: Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=32693284 #FOAvip The presence of SARS-CoV-2 in raw wastewaters has been demonstrated in many countries affected by this pandemic. Nevertheless, virus presence and infectivity in treated wastewaters, but also in the receiving water bodies are still poorly investigated. In this study, raw and treated samples from three wastewater treatment plants, and three river samples within the Milano Metropolitan Area, Italy, were surveyed for SARS-CoV-2 RNA detection by means of real time RT-PCR and infectivity test on culture cells. SARS-CoV-2 RNA was detected in raw, but not in treated wastewaters (four and two samples, respectively, sampled in two dates). The isolated virus genome was sequenced, and belonged to the strain most spread in Europe and similar to another found in the same region. RNA presence in raw wastewater samples decreased after eight days, probably following the epidemiological trend estimated for the area. Virus infectivity was always null, indicating the natural decay of viral pathogenicity in time from emission. Samples from receiving rivers (three sites, sampled in the same dates as wastewaters) showed in some cases a positivity to real time RT-PCR, probably due to non-treated, or inefficiently treated discharges, or to the combined sewage overflows. Nevertheless, also for rivers infectivity was null. Risks for public health should be limited, although a precautionary approach to risk assessment is here advocated, giving the preliminary nature of the presented data. Twit Text FOAVip Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers ????Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=32693284 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32693284 #FOAvip English Wikipedia <ref>{{Cite pmid|32693284}}</ref> Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers #MMPMID32693284 Rimoldi SG; Stefani F; Gigantiello A; Polesello S; Comandatore F; Mileto D; Maresca M; Longobardi C; Mancon A; Romeri F; Pagani C; Cappelli F; Roscioli C; Moja L; Gismondo MR; Salerno F Sci Total Environ 2020[Nov]; 744 (ä): 140911 PMID32693284show ga The presence of SARS-CoV-2 in raw wastewaters has been demonstrated in many countries affected by this pandemic. Nevertheless, virus presence and infectivity in treated wastewaters, but also in the receiving water bodies are still poorly investigated. In this study, raw and treated samples from three wastewater treatment plants, and three river samples within the Milano Metropolitan Area, Italy, were surveyed for SARS-CoV-2 RNA detection by means of real time RT-PCR and infectivity test on culture cells. SARS-CoV-2 RNA was detected in raw, but not in treated wastewaters (four and two samples, respectively, sampled in two dates). The isolated virus genome was sequenced, and belonged to the strain most spread in Europe and similar to another found in the same region. RNA presence in raw wastewater samples decreased after eight days, probably following the epidemiological trend estimated for the area. Virus infectivity was always null, indicating the natural decay of viral pathogenicity in time from emission. Samples from receiving rivers (three sites, sampled in the same dates as wastewaters) showed in some cases a positivity to real time RT-PCR, probably due to non-treated, or inefficiently treated discharges, or to the combined sewage overflows. Nevertheless, also for rivers infectivity was null. Risks for public health should be limited, although a precautionary approach to risk assessment is here advocated, giving the preliminary nature of the presented data. |*Coronavirus Infections[MESH] |*Pandemics[MESH] |*Pneumonia, Viral[MESH] |*Rivers[MESH] |*Wastewater[MESH] |Betacoronavirus[MESH] |COVID-19[MESH] |Europe[MESH] |Humans[MESH] |Italy[MESH]Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers(epmc).pdf DeepDyve Pubget Overpricing