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10.1084/jem.20201181 http://scihub22266oqcxt.onion/10.1084/jem.20201181 32692348!7372514!32692348     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Exp+Med 2020 ; 217 (11): ä Nephropedia Template TP {{tp|p=32692348|t=2020. Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses |pdf=|usr=}}{{32692348}} gab.com Text Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses JO: J Exp Med http://www.kidney.de/pget.php?&tw=1&pmid=32692348 #FOAvip The emergence of SARS-CoV-2 and the ensuing explosive epidemic of COVID-19 disease has generated a need for assays to rapidly and conveniently measure the antiviral activity of SARS-CoV-2-specific antibodies. Here, we describe a collection of approaches based on SARS-CoV-2 spike-pseudotyped, single-cycle, replication-defective human immunodeficiency virus type-1 (HIV-1), and vesicular stomatitis virus (VSV), as well as a replication-competent VSV/SARS-CoV-2 chimeric virus. While each surrogate virus exhibited subtle differences in the sensitivity with which neutralizing activity was detected, the neutralizing activity of both convalescent plasma and human monoclonal antibodies measured using each virus correlated quantitatively with neutralizing activity measured using an authentic SARS-CoV-2 neutralization assay. The assays described herein are adaptable to high throughput and are useful tools in the evaluation of serologic immunity conferred by vaccination or prior SARS-CoV-2 infection, as well as the potency of convalescent plasma or human monoclonal antibodies. Twit Text FOAVip Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses ????J Exp Med http://www.kidney.de/pget.php?&tw=1&pmid=32692348 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32692348 #FOAvip English Wikipedia <ref>{{Cite pmid|32692348}}</ref> Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses #MMPMID32692348 Schmidt F; Weisblum Y; Muecksch F; Hoffmann HH; Michailidis E; Lorenzi JCC; Mendoza P; Rutkowska M; Bednarski E; Gaebler C; Agudelo M; Cho A; Wang Z; Gazumyan A; Cipolla M; Caskey M; Robbiani DF; Nussenzweig MC; Rice CM; Hatziioannou T; Bieniasz PD J Exp Med 2020[Nov]; 217 (11): ä PMID32692348show ga The emergence of SARS-CoV-2 and the ensuing explosive epidemic of COVID-19 disease has generated a need for assays to rapidly and conveniently measure the antiviral activity of SARS-CoV-2-specific antibodies. Here, we describe a collection of approaches based on SARS-CoV-2 spike-pseudotyped, single-cycle, replication-defective human immunodeficiency virus type-1 (HIV-1), and vesicular stomatitis virus (VSV), as well as a replication-competent VSV/SARS-CoV-2 chimeric virus. While each surrogate virus exhibited subtle differences in the sensitivity with which neutralizing activity was detected, the neutralizing activity of both convalescent plasma and human monoclonal antibodies measured using each virus correlated quantitatively with neutralizing activity measured using an authentic SARS-CoV-2 neutralization assay. The assays described herein are adaptable to high throughput and are useful tools in the evaluation of serologic immunity conferred by vaccination or prior SARS-CoV-2 infection, as well as the potency of convalescent plasma or human monoclonal antibodies. |Animals[MESH] |Antibodies, Monoclonal/immunology[MESH] |Antibodies, Neutralizing/*analysis/blood[MESH] |Antibodies, Viral/*analysis/blood[MESH] |Betacoronavirus/genetics/*immunology[MESH] |COVID-19[MESH] |Cell Line[MESH] |Chimera/genetics/immunology[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/*immunology/virology[MESH] |HEK293 Cells[MESH] |HIV-1/genetics/immunology[MESH] |Humans[MESH] |Immunoassay/*methods[MESH] |Neutralization Tests/methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/*immunology/virology[MESH] |Recombination, Genetic[MESH] |SARS-CoV-2[MESH] |Spike Glycoprotein, Coronavirus/genetics/immunology[MESH] |Vero Cells[MESH]Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped (epmc).pdf DeepDyve Pubget Overpricing