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10.1080/22221751.2020.1799723 http://scihub22266oqcxt.onion/10.1080/22221751.2020.1799723 32691695!7473213!32691695     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32691695&cmd=llinks): Failed to open stream: HTTP request failed! 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Dysregulation in Akt/mTOR/HIF-1 signaling identified by proteo-transcriptomics of SARS-CoV-2 infected cells |pdf=|usr=}}{{32691695}} gab.com Text Dysregulation in Akt/mTOR/HIF-1 signaling identified by proteo-transcriptomics of SARS-CoV-2 infected cells JO: Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32691695 #FOAvip How severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infections engage cellular host pathways and innate immunity in infected cells remains largely elusive. We performed an integrative proteo-transcriptomics analysis in SARS-CoV-2 infected Huh7 cells to map the cellular response to the invading virus over time. We identified four pathways, ErbB, HIF-1, mTOR and TNF signaling, among others that were markedly modulated during the course of the SARS-CoV-2 infection in vitro. Western blot validation of the downstream effector molecules of these pathways revealed a dose-dependent activation of Akt, mTOR, S6K1 and 4E-BP1 at 24 hours post infection (hpi). However, we found a significant inhibition of HIF-1alpha through 24hpi and 48hpi of the infection, suggesting a crosstalk between the SARS-CoV-2 and the Akt/mTOR/HIF-1 signaling pathways. Inhibition of the mTOR signaling pathway using Akt inhibitor MK-2206 showed a significant reduction in virus production. Further investigations are required to better understand the molecular sequelae in order to guide potential therapy in the management of severe coronavirus disease 2019 (COVID-19) patients. Twit Text FOAVip Dysregulation in Akt/mTOR/HIF-1 signaling identified by proteo-transcriptomics of SARS-CoV-2 infected cells ????Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32691695 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32691695 #FOAvip English Wikipedia <ref>{{Cite pmid|32691695}}</ref> Dysregulation in Akt/mTOR/HIF-1 signaling identified by proteo-transcriptomics of SARS-CoV-2 infected cells #MMPMID32691695 Appelberg S; Gupta S; Svensson Akusjarvi S; Ambikan AT; Mikaeloff F; Saccon E; Vegvari A; Benfeitas R; Sperk M; Stahlberg M; Krishnan S; Singh K; Penninger JM; Mirazimi A; Neogi U Emerg Microbes Infect 2020[Dec]; 9 (1): 1748-1760 PMID32691695show ga How severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infections engage cellular host pathways and innate immunity in infected cells remains largely elusive. We performed an integrative proteo-transcriptomics analysis in SARS-CoV-2 infected Huh7 cells to map the cellular response to the invading virus over time. We identified four pathways, ErbB, HIF-1, mTOR and TNF signaling, among others that were markedly modulated during the course of the SARS-CoV-2 infection in vitro. Western blot validation of the downstream effector molecules of these pathways revealed a dose-dependent activation of Akt, mTOR, S6K1 and 4E-BP1 at 24 hours post infection (hpi). However, we found a significant inhibition of HIF-1alpha through 24hpi and 48hpi of the infection, suggesting a crosstalk between the SARS-CoV-2 and the Akt/mTOR/HIF-1 signaling pathways. Inhibition of the mTOR signaling pathway using Akt inhibitor MK-2206 showed a significant reduction in virus production. Further investigations are required to better understand the molecular sequelae in order to guide potential therapy in the management of severe coronavirus disease 2019 (COVID-19) patients. |*Signal Transduction[MESH] |Betacoronavirus/*pathogenicity[MESH] |COVID-19[MESH] |Cell Line[MESH] |Chromatography, Liquid[MESH] |Coronavirus Infections/genetics/metabolism/*virology[MESH] |Gene Expression Profiling/*methods[MESH] |Gene Expression Regulation[MESH] |High-Throughput Nucleotide Sequencing[MESH] |Humans[MESH] |Hypoxia-Inducible Factor 1/genetics/metabolism[MESH] |Pandemics[MESH] |Pneumonia, Viral/genetics/metabolism/*virology[MESH] |Proteomics/*methods[MESH] |Proto-Oncogene Proteins c-akt/genetics/metabolism[MESH] |SARS-CoV-2[MESH] |TOR Serine-Threonine Kinases/genetics/metabolism[MESH]Dysregulation in Akt/mTOR/HIF-1 signaling identified by proteo-transcr(epmc).pdf DeepDyve Pubget Overpricing