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10.1016/j.watres.2020.116112 http://scihub22266oqcxt.onion/10.1016/j.watres.2020.116112 32688150!7347499!32688150     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Water+Res 2020 ; 184 (ä): 116112 Nephropedia Template TP {{tp|p=32688150|t=2020. Metagenomic water quality monitoring with a portable laboratory |pdf=|usr=}}{{32688150}} gab.com Text Metagenomic water quality monitoring with a portable laboratory JO: Water Res http://www.kidney.de/pget.php?&tw=1&pmid=32688150 #FOAvip We describe the technical feasibility of metagenomic water quality analysis using only portable equipment, for example mini-vacuum pumps and filtration units, mini-centrifuges, mini-PCR machines and the memory-stick sized MinION of Oxford Nanopore Technologies, for the library preparation and sequencing of 16S rRNA gene amplicons. Using this portable toolbox on site, we successfully characterized the microbiome of water samples collected from Birtley Sewage Treatment Plant, UK, and its environs. We also demonstrated the applicability of the portable metagenomics toolbox in a low-income country by surveying water samples from the Akaki River around Addis Ababa, Ethiopia. The 16S rRNA gene sequencing workflow, including DNA extraction, PCR amplification, sequencing library preparation, and sequencing was accomplished within one working day. The metagenomic data became available within 24-72 h, depending on internet speed. Metagenomic analysis clearly distinguished the microbiome of pristine samples from sewage influenced water samples. Metagenomic analysis identified the potential role of two bacterial genera not conventionally monitored, Arcobacter and Aeromonas, as predominant faecal pollution indicators/waterborne hazards. Subsequent quantitative PCR analysis validated the high Arcobacter butzleri abundances observed in the urban influenced Akaki River water samples by portable next generation sequencing with the MinION device. Overall, our field deployable metagenomics toolbox advances the capability of scientists to comprehensively monitor microbiomes anywhere in the world, including in the water, food and drinks industries, the health services, agriculture and beyond. Twit Text FOAVip Metagenomic water quality monitoring with a portable laboratory ????Water Res http://www.kidney.de/pget.php?&tw=1&pmid=32688150 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32688150 #FOAvip English Wikipedia <ref>{{Cite pmid|32688150}}</ref> Metagenomic water quality monitoring with a portable laboratory #MMPMID32688150 Acharya K; Blackburn A; Mohammed J; Haile AT; Hiruy AM; Werner D Water Res 2020[Oct]; 184 (ä): 116112 PMID32688150show ga We describe the technical feasibility of metagenomic water quality analysis using only portable equipment, for example mini-vacuum pumps and filtration units, mini-centrifuges, mini-PCR machines and the memory-stick sized MinION of Oxford Nanopore Technologies, for the library preparation and sequencing of 16S rRNA gene amplicons. Using this portable toolbox on site, we successfully characterized the microbiome of water samples collected from Birtley Sewage Treatment Plant, UK, and its environs. We also demonstrated the applicability of the portable metagenomics toolbox in a low-income country by surveying water samples from the Akaki River around Addis Ababa, Ethiopia. The 16S rRNA gene sequencing workflow, including DNA extraction, PCR amplification, sequencing library preparation, and sequencing was accomplished within one working day. The metagenomic data became available within 24-72 h, depending on internet speed. Metagenomic analysis clearly distinguished the microbiome of pristine samples from sewage influenced water samples. Metagenomic analysis identified the potential role of two bacterial genera not conventionally monitored, Arcobacter and Aeromonas, as predominant faecal pollution indicators/waterborne hazards. Subsequent quantitative PCR analysis validated the high Arcobacter butzleri abundances observed in the urban influenced Akaki River water samples by portable next generation sequencing with the MinION device. Overall, our field deployable metagenomics toolbox advances the capability of scientists to comprehensively monitor microbiomes anywhere in the world, including in the water, food and drinks industries, the health services, agriculture and beyond. |*Water[MESH] |*Water Quality[MESH] |Ethiopia[MESH] |High-Throughput Nucleotide Sequencing[MESH] |Metagenome[MESH] |Metagenomics[MESH]Metagenomic water quality monitoring with a portable laboratory (epmc).pdf DeepDyve Pubget Overpricing