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10.1007/s10096-020-03983-9

http://scihub22266oqcxt.onion/10.1007/s10096-020-03983-9
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32681309!7367510!32681309
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suck abstract from ncbi


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pmid32681309      Eur+J+Clin+Microbiol+Infect+Dis 2020 ; 39 (12): 2373-2377
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  • Variplex test system fails to reliably detect SARS-CoV-2 directly from respiratory samples without RNA extraction #MMPMID32681309
  • Eckel F; Kusters F; Drossel B; Konert M; Mattes H; Schopf S
  • Eur J Clin Microbiol Infect Dis 2020[Dec]; 39 (12): 2373-2377 PMID32681309show ga
  • Diagnosis of COVID is performed by PCR methods, but their capacity is limited by the requirement of high-level facilities and instruments. The loop-mediated isothermal amplification (LAMP) method has been utilized for the detection of isolated virus-specific RNA. Preliminary data suggest the possibility of isothermal amplification directly from respiratory samples without RNA extraction. All patients admitted to our hospital were screened for SARS-CoV-2 by routine. Respiratory samples were tested by variplex system based on LAMP method directly without RNA extraction and by PCR. Primary endpoint was the false-negative rate of variplex test compared with PCR as gold standard. In 109 patients variplex test and PCR assay were performed simultaneously. Median age was 80 years and male/female ratio was 40/60%. The prevalence of PCR-confirmed COVID diagnosis was 43.1%. Variplex test was positive in 13.8%. False-negative rate of variplex test compared with PCR was 83.0%. The potential of LAMP technology using isolated RNA has been demonstrated impressively by others, and excellent sensitivity and specificity of detecting SARS-CoV-2 has been reported. However, without RNA extraction, the variplex test system failed to reliably detect SARS-CoV-2 directly in respiratory samples.
  • |*Pandemics[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Betacoronavirus/*genetics/pathogenicity[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques/*methods[MESH]
  • |Coronavirus Infections/blood/*diagnosis/epidemiology/mortality[MESH]
  • |False Negative Reactions[MESH]
  • |Female[MESH]
  • |Germany/epidemiology[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Molecular Diagnostic Techniques/*methods[MESH]
  • |Nasopharynx/virology[MESH]
  • |Nucleic Acid Amplification Techniques/*methods[MESH]
  • |Oropharynx/virology[MESH]
  • |Pneumonia, Viral/blood/*diagnosis/epidemiology/mortality[MESH]
  • |RNA, Viral/*blood/genetics[MESH]
  • |Reagent Kits, Diagnostic/standards[MESH]
  • |Retrospective Studies[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction/standards[MESH]
  • |SARS-CoV-2[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Severity of Illness Index[MESH]


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