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10.1590/0074-02760200200

http://scihub22266oqcxt.onion/10.1590/0074-02760200200
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32667461!7357545!32667461
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suck abstract from ncbi


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pmid32667461      Mem+Inst+Oswaldo+Cruz 2020 ; 115 (ä): e200200
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  • SARS-CoV-2 diagnostic diary: from rumors to the first case Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro #MMPMID32667461
  • Cipitelli MDC; Valentin E; da Cruz NVG; Nogueira TL; de Melo ECA; da Silva RA; Serra MM; Meriano AL; Colares AM; Dornelas-Ribeiro M; Santos CG
  • Mem Inst Oswaldo Cruz 2020[]; 115 (ä): e200200 PMID32667461show ga
  • Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charite protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.
  • |Betacoronavirus/*genetics/isolation & purification[MESH]
  • |Brazil/epidemiology[MESH]
  • |COVID-19[MESH]
  • |Coronavirus Infections/*diagnosis/epidemiology[MESH]
  • |Humans[MESH]
  • |Military Facilities[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/epidemiology[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction[MESH]


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