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10.1007/s11033-020-05658-2 http://scihub22266oqcxt.onion/10.1007/s11033-020-05658-2 32661869!7356131!32661869     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Biol+Rep 2020 ; 47 (8): 5879-5887 Nephropedia Template TP {{tp|p=32661869|t=2020. Efficiency and cell viability implications using tip type electroporation in zebrafish sperm cells |pdf=|usr=}}{{32661869}} gab.com Text Efficiency and cell viability implications using tip type electroporation in zebrafish sperm cells JO: Mol Biol Rep http://www.kidney.de/pget.php?&tw=1&pmid=32661869 #FOAvip Sperm-mediated gene transfer (SMGT) has a potential use for zebrafish transgenesis. However, transfection into fish sperm cells still needs to be improved. The objective was to demonstrate the feasibility of tip type electroporation in zebrafish sperm, showing a protocol that provide high transfection efficiency, with minimal side-effects. Sperm was transfected with a Cy3-labelled DNA using tip type electroporation with voltages ranging from 500 to 1500 V. Sperm kinetics parameters were assessed using Computer Assisted Semen Analysis (CASA) and cell integrity, reactive oxygen species (ROS), mitochondrial functionality and transfection rate were evaluated by flow cytometry. The transfection rates were positively affected by tip type electroporation, reaching 64.9% +/- 3.6 in the lowest voltage used (500 V) and 86.6% +/- 1.9 in the highest (1500 V). The percentage of overall motile sperm in the electrotransfected samples was found to decrease with increasing field strength (P < 0.05). Increase in the sperm damaged plasma membrane was observed with increasing field strength (P < 0.05). ROS and sperm mitochondrial functionality did not present a negative response after the electroporation (P > 0.05). Overall results indicate that tip type electroporation enhances the internalization of exogenous DNA into zebrafish sperm cells with minimal harmful effects to sperm cells. Twit Text FOAVip Efficiency and cell viability implications using tip type electroporation in zebrafish sperm cells ????Mol Biol Rep http://www.kidney.de/pget.php?&tw=1&pmid=32661869 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32661869 #FOAvip English Wikipedia <ref>{{Cite pmid|32661869}}</ref> Efficiency and cell viability implications using tip type electroporation in zebrafish sperm cells #MMPMID32661869 Daneluz LO; Acosta IB; Nunes LS; Blodorn EB; Domingues WB; Martins AWS; Dellagostin EN; Rassier GT; Corcini CD; Froes CN; Komninou ER; Varela AS Jr; Campos VF Mol Biol Rep 2020[Aug]; 47 (8): 5879-5887 PMID32661869show ga Sperm-mediated gene transfer (SMGT) has a potential use for zebrafish transgenesis. However, transfection into fish sperm cells still needs to be improved. The objective was to demonstrate the feasibility of tip type electroporation in zebrafish sperm, showing a protocol that provide high transfection efficiency, with minimal side-effects. Sperm was transfected with a Cy3-labelled DNA using tip type electroporation with voltages ranging from 500 to 1500 V. Sperm kinetics parameters were assessed using Computer Assisted Semen Analysis (CASA) and cell integrity, reactive oxygen species (ROS), mitochondrial functionality and transfection rate were evaluated by flow cytometry. The transfection rates were positively affected by tip type electroporation, reaching 64.9% +/- 3.6 in the lowest voltage used (500 V) and 86.6% +/- 1.9 in the highest (1500 V). The percentage of overall motile sperm in the electrotransfected samples was found to decrease with increasing field strength (P < 0.05). Increase in the sperm damaged plasma membrane was observed with increasing field strength (P < 0.05). ROS and sperm mitochondrial functionality did not present a negative response after the electroporation (P > 0.05). Overall results indicate that tip type electroporation enhances the internalization of exogenous DNA into zebrafish sperm cells with minimal harmful effects to sperm cells. |Animals[MESH] |Cell Survival[MESH] |DNA/*administration & dosage[MESH] |Electroporation/*methods[MESH] |Fertilization in Vitro/methods[MESH] |Gene Transfer Techniques[MESH] |Male[MESH] |Mitochondria/*physiology[MESH] |Reactive Oxygen Species/*metabolism[MESH] |Sperm Motility[MESH] |Spermatozoa/*physiology[MESH] |Transfection/methods[MESH]Efficiency and cell viability implications using tip type electroporat(epmc).pdf DeepDyve Pubget Overpricing