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10.1038/s41582-020-0374-y http://scihub22266oqcxt.onion/10.1038/s41582-020-0374-y 32661342!7356134!32661342     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32661342&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Rev+Neurol 2020 ; 16 (10): 547-556 Nephropedia Template TP {{tp|p=32661342|t=2020. Metagenomics for neurological infections - expanding our imagination |pdf=|usr=}}{{32661342}} gab.com Text Metagenomics for neurological infections - expanding our imagination JO: Nat Rev Neurol http://www.kidney.de/pget.php?&tw=1&pmid=32661342 #FOAvip Over the past two decades, the diagnosis rate for patients with encephalitis has remained poor despite advances in pathogen-specific testing such as PCR and antigen assays. Metagenomic next-generation sequencing (mNGS) of RNA and DNA extracted from cerebrospinal fluid and brain tissue now offers another strategy for diagnosing neurological infections. Given that mNGS simultaneously assays for a wide range of infectious agents in an unbiased manner, it can identify pathogens that were not part of a neurologist's initial differential diagnosis either because of the rarity of the infection, because the microorganism has not been previously associated with a clinical phenotype or because it is a newly discovered organism. This Review discusses the technical advantages and pitfalls of cerebrospinal fluid mNGS in the context of patients with neuroinflammatory syndromes, including encephalitis, meningitis and myelitis. We also speculate on how mNGS testing potentially fits into current diagnostic testing algorithms given data on mNGS test performance, cost and turnaround time. Finally, the Review highlights future directions for mNGS technology and other hypothesis-free testing methodologies that are in development. Twit Text FOAVip Metagenomics for neurological infections - expanding our imagination ????Nat Rev Neurol http://www.kidney.de/pget.php?&tw=1&pmid=32661342 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32661342 #FOAvip English Wikipedia <ref>{{Cite pmid|32661342}}</ref> Metagenomics for neurological infections - expanding our imagination #MMPMID32661342 Ramachandran PS; Wilson MR Nat Rev Neurol 2020[Oct]; 16 (10): 547-556 PMID32661342show ga Over the past two decades, the diagnosis rate for patients with encephalitis has remained poor despite advances in pathogen-specific testing such as PCR and antigen assays. Metagenomic next-generation sequencing (mNGS) of RNA and DNA extracted from cerebrospinal fluid and brain tissue now offers another strategy for diagnosing neurological infections. Given that mNGS simultaneously assays for a wide range of infectious agents in an unbiased manner, it can identify pathogens that were not part of a neurologist's initial differential diagnosis either because of the rarity of the infection, because the microorganism has not been previously associated with a clinical phenotype or because it is a newly discovered organism. This Review discusses the technical advantages and pitfalls of cerebrospinal fluid mNGS in the context of patients with neuroinflammatory syndromes, including encephalitis, meningitis and myelitis. We also speculate on how mNGS testing potentially fits into current diagnostic testing algorithms given data on mNGS test performance, cost and turnaround time. Finally, the Review highlights future directions for mNGS technology and other hypothesis-free testing methodologies that are in development. |*Imagination[MESH] |Animals[MESH] |Central Nervous System Infections/*diagnosis/*genetics/therapy[MESH] |Computational Biology/methods/trends[MESH] |Data Interpretation, Statistical[MESH] |Encephalitis/diagnosis/genetics/therapy[MESH] |Humans[MESH]Metagenomics for neurological infections - expanding our imagination (epmc).pdf DeepDyve Pubget Overpricing