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10.1021/acssensors.0c00927

http://scihub22266oqcxt.onion/10.1021/acssensors.0c00927
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32660240!ä!32660240

suck abstract from ncbi


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pmid32660240      ACS+Sens 2020 ; 5 (8): 2331-2337
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  • Development of a Sensitive Immunochromatographic Method Using Lanthanide Fluorescent Microsphere for Rapid Serodiagnosis of COVID-19 #MMPMID32660240
  • Feng M; Chen J; Xun J; Dai R; Zhao W; Lu H; Xu J; Chen L; Sui G; Cheng X
  • ACS Sens 2020[Aug]; 5 (8): 2331-2337 PMID32660240show ga
  • The SARS-CoV-2 infection that caused the COVID-19 pandemic quickly spread worldwide within two months. Rapid diagnosis of the disease and isolation of patients are effective ways to prevent and control the spread of COVID-19. Therefore, a sensitive immunofluorescent assay method was developed for rapid detection of special IgM and IgG of COVID-19 in human serum within 10 min. The recombinant nucleocapsid protein of 2019 novel coronavirus was used as capture antigen. Lanthanide, Eu(III) fluorescent microsphere, was used to qualitatively/semiquantitatively determine the solid phase immunochromatographic assay. A total of 28 clinical positive and 77 negative serum or plasma samples were included in the test. Based on the analysis of serum or plasma from COVID-19 patients and healthy people, the sensitivity and specificity of the immunochromatographic assay were calculated as 98.72% and 100% (IgG), and 98.68% and 93.10% (IgM), respectively. The results demonstrated that rapid immunoassay has high sensitivity and specificity and was useful for rapid serodiagnosis of COVID-19.
  • |*Microspheres[MESH]
  • |COVID-19[MESH]
  • |Coronavirus Infections/*diagnosis[MESH]
  • |Europium[MESH]
  • |Fluorescence[MESH]
  • |Humans[MESH]
  • |Immunoassay/*methods[MESH]
  • |Immunoglobulin G/blood[MESH]
  • |Immunoglobulin M/blood[MESH]
  • |Lanthanoid Series Elements[MESH]
  • |Nucleocapsid Proteins/immunology[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Serologic Tests/*methods/standards[MESH]


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