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10.1016/j.jcv.2020.104541 http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104541 32659713!7336924!32659713     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2020 ; 129 (ä): 104541 Nephropedia Template TP {{tp|p=32659713|t=2020. Evaluation of the Aptima transcription-mediated amplification assay (Hologic(R)) for detecting SARS-CoV-2 in clinical specimens |pdf=|usr=}}{{32659713}} gab.com Text Evaluation of the Aptima transcription-mediated amplification assay (Hologic(R)) for detecting SARS-CoV-2 in clinical specimens JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32659713 #FOAvip BACKGROUND: The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which appeared in late 2019, has been limited by isolating infected individuals. However, identifying such individuals requires accurate diagnostic tools. OBJECTIVE: This study evaluates the capacity of the Aptima Transcription-Mediated Amplification (TMA) assay (Hologic(R) Panther System) to detect the virus in clinical samples. STUDY DESIGN: We compared the Aptima assay to two in-house real-time RT-PCR techniques, one running on the Panther Fusion module and the other on the MagNA Pure 96 and Light-Cycler 480 instruments. We included a total of 200 respiratory specimens: 100 tested prospectively and 100 retrospectively (25 -ve/75 +ve). RESULTS: The final Cohen's kappa coefficients were: kappa?=?0.978 between the Aptima and Panther Fusion assays, kappa?=?0.945 between the Aptima and MagNA/LC480 assays and kappa?=?0.956 between the MagNA/LC480 and Panther Fusion assays. CONCLUSION: These findings indicate that the Aptima SARS-CoV-2 TMA assay data agree well with those obtained with our routine methods and that this assay can be used to diagnose coronavirus disease 2019 (COVID-19). Twit Text FOAVip Evaluation of the Aptima transcription-mediated amplification assay (Hologic(R)) for detecting SARS-CoV-2 in clinical specimens ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32659713 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32659713 #FOAvip English Wikipedia <ref>{{Cite pmid|32659713}}</ref> Evaluation of the Aptima transcription-mediated amplification assay (Hologic(R)) for detecting SARS-CoV-2 in clinical specimens #MMPMID32659713 Tremeaux P; Lhomme S; Abravanel F; Raymond S; Mengelle C; Mansuy JM; Izopet J J Clin Virol 2020[Aug]; 129 (ä): 104541 PMID32659713show ga BACKGROUND: The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which appeared in late 2019, has been limited by isolating infected individuals. However, identifying such individuals requires accurate diagnostic tools. OBJECTIVE: This study evaluates the capacity of the Aptima Transcription-Mediated Amplification (TMA) assay (Hologic(R) Panther System) to detect the virus in clinical samples. STUDY DESIGN: We compared the Aptima assay to two in-house real-time RT-PCR techniques, one running on the Panther Fusion module and the other on the MagNA Pure 96 and Light-Cycler 480 instruments. We included a total of 200 respiratory specimens: 100 tested prospectively and 100 retrospectively (25 -ve/75 +ve). RESULTS: The final Cohen's kappa coefficients were: kappa?=?0.978 between the Aptima and Panther Fusion assays, kappa?=?0.945 between the Aptima and MagNA/LC480 assays and kappa?=?0.956 between the MagNA/LC480 and Panther Fusion assays. CONCLUSION: These findings indicate that the Aptima SARS-CoV-2 TMA assay data agree well with those obtained with our routine methods and that this assay can be used to diagnose coronavirus disease 2019 (COVID-19). |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Betacoronavirus/genetics/*isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |COVID-19 Vaccines[MESH] |Clinical Laboratory Techniques/*methods[MESH] |Coronavirus Infections/*diagnosis[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Nucleic Acid Amplification Techniques/*methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |Prospective Studies[MESH] |Retrospective Studies[MESH]Evaluation of the Aptima transcription-mediated amplification assay (H(epmc).pdf DeepDyve Pubget Overpricing