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10.1016/j.jviromet.2020.113937 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.113937 32659241!7351060!32659241     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2020 ; 284 (ä): 113937 Nephropedia Template TP {{tp|p=32659241|t=2020. Capillary electrophoresis of PCR fragments with 5 -labelled primers for testing the SARS-Cov-2 |pdf=|usr=}}{{32659241}} gab.com Text Capillary electrophoresis of PCR fragments with 5 -labelled primers for testing the SARS-Cov-2 JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=32659241 #FOAvip Due to the huge demand for SARS-Cov-2 determination,alternatives to the standard qtPCRtestsare potentially useful for increasing the number of samples screened. Our aim was to develop a direct fluorescent PCR capillary-electrophoresis detection of the viral genome. We validated this approach on several SARS-Cov-2 positive and negative samples.We isolated the naso-pharingealRNA from 20 positive and 10 negative samples. The cDNA was synthesised and two fragments of the SARS-Cov-2 were amplified. One of the primers for each pair was 5 -end fluorochrome labelled. The amplifications were subjected to capillary electrophoresis in ABI3130 sequencers to visualize the fluorescent peaks.The two SARS-Cov-2 fragments were successfully amplified in the positive samples, while the negative samples did not render fluorescent peaks. In conclusion, we describe and alternative method to identify the SARS-Cov-2 genome that could be scaled to the analysis of approximately 100 samples in less than 5 h. By combining a standard PCR with capillary electrophoresis our approach would overcome the limits imposed to many labs by the qtPCR and increase the testing capacity. Twit Text FOAVip Capillary electrophoresis of PCR fragments with 5 -labelled primers for testing the SARS-Cov-2 ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=32659241 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32659241 #FOAvip English Wikipedia <ref>{{Cite pmid|32659241}}</ref> Capillary electrophoresis of PCR fragments with 5 -labelled primers for testing the SARS-Cov-2 #MMPMID32659241 Gomez J; Melon S; Boga JA; Alvarez-Arguelles ME; Rojo-Alba S; Leal-Negredo A; Castello-Abietar C; Alvarez V; Cuesta-Llavona E; Coto E J Virol Methods 2020[Oct]; 284 (ä): 113937 PMID32659241show ga Due to the huge demand for SARS-Cov-2 determination,alternatives to the standard qtPCRtestsare potentially useful for increasing the number of samples screened. Our aim was to develop a direct fluorescent PCR capillary-electrophoresis detection of the viral genome. We validated this approach on several SARS-Cov-2 positive and negative samples.We isolated the naso-pharingealRNA from 20 positive and 10 negative samples. The cDNA was synthesised and two fragments of the SARS-Cov-2 were amplified. One of the primers for each pair was 5 -end fluorochrome labelled. The amplifications were subjected to capillary electrophoresis in ABI3130 sequencers to visualize the fluorescent peaks.The two SARS-Cov-2 fragments were successfully amplified in the positive samples, while the negative samples did not render fluorescent peaks. In conclusion, we describe and alternative method to identify the SARS-Cov-2 genome that could be scaled to the analysis of approximately 100 samples in less than 5 h. By combining a standard PCR with capillary electrophoresis our approach would overcome the limits imposed to many labs by the qtPCR and increase the testing capacity. |Base Sequence[MESH] |Betacoronavirus/genetics/*isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/*methods[MESH] |Coronavirus Infections/diagnosis/*virology[MESH] |DNA Primers/genetics[MESH] |DNA, Complementary/genetics[MESH] |Electrophoresis, Capillary/*methods[MESH] |Genome, Viral[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques/methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/diagnosis/*virology[MESH] |Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2[MESH]Capillary electrophoresis of PCR fragments with 5 -labelled primers fo(epmc).pdf DeepDyve Pubget Overpricing