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10.1111/jam.14781 http://scihub22266oqcxt.onion/10.1111/jam.14781 32652813!7405274!32652813     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32652813&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Appl+Microbiol 2021 ; 130 (1): 2-13 Nephropedia Template TP {{tp|p=32652813|t=2021. Comparative in silico design and validation of GPS CoVID-19 dtec-RT-qPCR test |pdf=|usr=}}{{32652813}} gab.com Text Comparative in silico design and validation of GPS CoVID-19 dtec-RT-qPCR test JO: J Appl Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=32652813 #FOAvip AIMS: Providing a ready-to-use reverse transcriptase qPCR (RT-qPCR) method fully validated to detect the SARS-CoV-2 with a higher exclusivity than this shown by early published RT-qPCR designs. METHODS AND RESULTS: The specificity of the GPS CoVID-19 dtec-RT-qPCR test by analysis of sequence alignments was approached and compared with other RT-qPCR designs. The GPS CoVID-19 dtec-RT-qPCR test was validated following criteria of UNE/EN ISO 17025:2005 and ISO/IEC 15189:2012. Diagnostic validation was achieved by two independent reference laboratories, the Instituto de Salud Carlos III, (Madrid, Spain), the Public Health England (Colindale, London, UK), and received the label CE-IVD. The GPS design showed the highest exclusivity and passed all parameters of validation with strict acceptance criteria. Results from reference laboratories 100% correlated with these obtained by using reference methods and showed 100% of diagnostic sensitivity and specificity. CONCLUSIONS: The CE-IVD GPS CoVID-19 dtec-RT-qPCR test, available worldwide with full analytical and diagnostic validation, is the more exclusive for SARS-CoV-2 by far. SIGNIFICANCE AND IMPACT OF THE STUDY: Considering the CoVID-19 pandemic status, the exclusivity of RT-qPCR tests is crucial to avoid false positives due to related coronaviruses. This work provides of a highly specific and validated RT-qPCR method for detection of SARS-CoV-2, which represents a case of efficient transfer of technology successfully used since the pandemic was declared. Twit Text FOAVip Comparative in silico design and validation of GPS CoVID-19 dtec-RT-qPCR test ????J Appl Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=32652813 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32652813 #FOAvip English Wikipedia <ref>{{Cite pmid|32652813}}</ref> Comparative in silico design and validation of GPS CoVID-19 dtec-RT-qPCR test #MMPMID32652813 Martinez-Murcia A; Bru G; Navarro A; Ros-Tarraga P; Garcia-Sirera A; Perez L J Appl Microbiol 2021[Jan]; 130 (1): 2-13 PMID32652813show ga AIMS: Providing a ready-to-use reverse transcriptase qPCR (RT-qPCR) method fully validated to detect the SARS-CoV-2 with a higher exclusivity than this shown by early published RT-qPCR designs. METHODS AND RESULTS: The specificity of the GPS CoVID-19 dtec-RT-qPCR test by analysis of sequence alignments was approached and compared with other RT-qPCR designs. The GPS CoVID-19 dtec-RT-qPCR test was validated following criteria of UNE/EN ISO 17025:2005 and ISO/IEC 15189:2012. Diagnostic validation was achieved by two independent reference laboratories, the Instituto de Salud Carlos III, (Madrid, Spain), the Public Health England (Colindale, London, UK), and received the label CE-IVD. The GPS design showed the highest exclusivity and passed all parameters of validation with strict acceptance criteria. Results from reference laboratories 100% correlated with these obtained by using reference methods and showed 100% of diagnostic sensitivity and specificity. CONCLUSIONS: The CE-IVD GPS CoVID-19 dtec-RT-qPCR test, available worldwide with full analytical and diagnostic validation, is the more exclusive for SARS-CoV-2 by far. SIGNIFICANCE AND IMPACT OF THE STUDY: Considering the CoVID-19 pandemic status, the exclusivity of RT-qPCR tests is crucial to avoid false positives due to related coronaviruses. This work provides of a highly specific and validated RT-qPCR method for detection of SARS-CoV-2, which represents a case of efficient transfer of technology successfully used since the pandemic was declared. |COVID-19 Nucleic Acid Testing/*methods/standards[MESH] |COVID-19/*diagnosis[MESH] |Computer Simulation[MESH] |Humans[MESH] |Pandemics[MESH] |Real-Time Polymerase Chain Reaction[MESH] |Reproducibility of Results[MESH] |SARS-CoV-2/classification/genetics/*isolation & purification[MESH] |Sensitivity and Specificity[MESH]Comparative in silico design and validation of GPS CoVID-19 dtec-RT-qP(epmc).pdf DeepDyve Pubget Overpricing