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10.1016/j.jcv.2020.104520 http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104520 32652476!7323686!32652476     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2020 ; 129 (ä): 104520 Nephropedia Template TP {{tp|p=32652476|t=2020. Evaluation of the RealStar(R) SARS-CoV-2 RT-PCR kit RUO performances and limit of detection |pdf=|usr=}}{{32652476}} gab.com Text Evaluation of the RealStar(R) SARS-CoV-2 RT-PCR kit RUO performances and limit of detection JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32652476 #FOAvip BACKGROUND: The use of efficient, reliable and sensitive PCR assays is a cornerstone in the race to contain the SARS-CoV-2 pandemic. In this work we performed an independent evaluation of the RealStar(R) SARS-CoV-2 RT-PCR Kit Researh Use Only (Altona) for SARS-CoV-2 detection. METHODS: A comparative limit of detection (LoD) assessment was performed between RealStar(R) SARS-CoV-2 and the currently WHO recommended RT-PCR (WHO-PCR) workflow using a quantified clinical sample. Assessment of the RealStar(R) SARS-CoV-2 assay was also performed using 83 primary clinical samples in comparison with the WHO-PCR. RESULTS: The RealStar(R) SARS-CoV-2 demonstrated a slightly higher sensitivity than the WHO recommended assay with a limit of detection at 625 copies/mL instead of 1250 copies/mL for the WHO-PCR in our conditions. The overall percent agreement between RealStar(R) SARS-CoV-2 and WHO-PCR on 83 clinical samples was 97.6 % (81/83) with a sensitivity at 97.8 % (45/46) and specificity at 97.3 % (36/37). No cross reaction was encountered for the other human coronaviruses (HKU1, OC43, NL63, 229E). CONCLUSIONS: In this comparison of the RealStar(R) SARS-CoV-2 assay with the reference WHO assay, we observed a slightly better sensitivity of the RealStar(R) assay. It provides a robust option for all molecular biology laboratories, with a strong real-life LoD and is compatible with various real-time PCR platforms. Twit Text FOAVip Evaluation of the RealStar(R) SARS-CoV-2 RT-PCR kit RUO performances and limit of detection ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32652476 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32652476 #FOAvip English Wikipedia <ref>{{Cite pmid|32652476}}</ref> Evaluation of the RealStar(R) SARS-CoV-2 RT-PCR kit RUO performances and limit of detection #MMPMID32652476 Visseaux B; Le Hingrat Q; Collin G; Ferre V; Storto A; Ichou H; Bouzid D; Poey N; de Montmollin E; Descamps D; Houhou-Fidouh N J Clin Virol 2020[Aug]; 129 (ä): 104520 PMID32652476show ga BACKGROUND: The use of efficient, reliable and sensitive PCR assays is a cornerstone in the race to contain the SARS-CoV-2 pandemic. In this work we performed an independent evaluation of the RealStar(R) SARS-CoV-2 RT-PCR Kit Researh Use Only (Altona) for SARS-CoV-2 detection. METHODS: A comparative limit of detection (LoD) assessment was performed between RealStar(R) SARS-CoV-2 and the currently WHO recommended RT-PCR (WHO-PCR) workflow using a quantified clinical sample. Assessment of the RealStar(R) SARS-CoV-2 assay was also performed using 83 primary clinical samples in comparison with the WHO-PCR. RESULTS: The RealStar(R) SARS-CoV-2 demonstrated a slightly higher sensitivity than the WHO recommended assay with a limit of detection at 625 copies/mL instead of 1250 copies/mL for the WHO-PCR in our conditions. The overall percent agreement between RealStar(R) SARS-CoV-2 and WHO-PCR on 83 clinical samples was 97.6 % (81/83) with a sensitivity at 97.8 % (45/46) and specificity at 97.3 % (36/37). No cross reaction was encountered for the other human coronaviruses (HKU1, OC43, NL63, 229E). CONCLUSIONS: In this comparison of the RealStar(R) SARS-CoV-2 assay with the reference WHO assay, we observed a slightly better sensitivity of the RealStar(R) assay. It provides a robust option for all molecular biology laboratories, with a strong real-life LoD and is compatible with various real-time PCR platforms. |Betacoronavirus/*isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |COVID-19 Vaccines[MESH] |Clinical Laboratory Techniques/*methods[MESH] |Coronavirus Infections/*diagnosis[MESH] |Humans[MESH] |Limit of Detection[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2[MESH]Evaluation of the RealStar(R) SARS-CoV-2 RT-PCR kit RUO performances a(epmc).pdf DeepDyve Pubget Overpricing