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10.1038/s41564-020-0761-6 http://scihub22266oqcxt.onion/10.1038/s41564-020-0761-6 32651556!9241364!32651556     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Microbiol 2020 ; 5 (10): 1299-1305 Nephropedia Template TP {{tp|p=32651556|t=2020. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets |pdf=|usr=}}{{32651556}} gab.com Text Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets JO: Nat Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=32651556 #FOAvip The recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for accurate and rapid diagnostic assays to prompt clinical and public health interventions. Currently, several quantitative reverse transcription-PCR (RT-qPCR) assays are being used by clinical, research and public health laboratories. However, it is currently unclear whether results from different tests are comparable. Our goal was to make independent evaluations of primer-probe sets used in four common SARS-CoV-2 diagnostic assays. From our comparisons of RT-qPCR analytical efficiency and sensitivity, we show that all primer-probe sets can be used to detect SARS-CoV-2 at 500 viral RNA copies per reaction. The exception for this is the RdRp-SARSr (Charite) confirmatory primer-probe set which has low sensitivity, probably due to a mismatch to circulating SARS-CoV-2 in the reverse primer. We did not find evidence for background amplification with pre-COVID-19 samples or recent SARS-CoV-2 evolution decreasing sensitivity. Our recommendation for SARS-CoV-2 diagnostic testing is to select an assay with high sensitivity and that is regionally used, to ease comparability between outcomes. Twit Text FOAVip Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets ????Nat Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=32651556 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32651556 #FOAvip English Wikipedia <ref>{{Cite pmid|32651556}}</ref> Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets #MMPMID32651556 Vogels CBF; Brito AF; Wyllie AL; Fauver JR; Ott IM; Kalinich CC; Petrone ME; Casanovas-Massana A; Catherine Muenker M; Moore AJ; Klein J; Lu P; Lu-Culligan A; Jiang X; Kim DJ; Kudo E; Mao T; Moriyama M; Oh JE; Park A; Silva J; Song E; Takahashi T; Taura M; Tokuyama M; Venkataraman A; Weizman OE; Wong P; Yang Y; Cheemarla NR; White EB; Lapidus S; Earnest R; Geng B; Vijayakumar P; Odio C; Fournier J; Bermejo S; Farhadian S; Dela Cruz CS; Iwasaki A; Ko AI; Landry ML; Foxman EF; Grubaugh ND Nat Microbiol 2020[Oct]; 5 (10): 1299-1305 PMID32651556show ga The recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for accurate and rapid diagnostic assays to prompt clinical and public health interventions. Currently, several quantitative reverse transcription-PCR (RT-qPCR) assays are being used by clinical, research and public health laboratories. However, it is currently unclear whether results from different tests are comparable. Our goal was to make independent evaluations of primer-probe sets used in four common SARS-CoV-2 diagnostic assays. From our comparisons of RT-qPCR analytical efficiency and sensitivity, we show that all primer-probe sets can be used to detect SARS-CoV-2 at 500 viral RNA copies per reaction. The exception for this is the RdRp-SARSr (Charite) confirmatory primer-probe set which has low sensitivity, probably due to a mismatch to circulating SARS-CoV-2 in the reverse primer. We did not find evidence for background amplification with pre-COVID-19 samples or recent SARS-CoV-2 evolution decreasing sensitivity. Our recommendation for SARS-CoV-2 diagnostic testing is to select an assay with high sensitivity and that is regionally used, to ease comparability between outcomes. |Betacoronavirus/*genetics/isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/*methods/statistics & numerical data[MESH] |Coronavirus Infections/*diagnosis/epidemiology/*virology[MESH] |Genetic Variation[MESH] |Genome, Viral[MESH] |Humans[MESH] |Molecular Probe Techniques/statistics & numerical data[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/epidemiology/*virology[MESH] |RNA Probes/genetics[MESH] |RNA, Viral/*analysis/*genetics[MESH] |RNA/genetics[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods/statistics & numerical data[MESH] |SARS-CoV-2[MESH]Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPC(epmc).pdf DeepDyve Pubget Overpricing