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10.1016/j.fertnstert.2020.05.028 http://scihub22266oqcxt.onion/10.1016/j.fertnstert.2020.05.028 32650948!7256599!32650948     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Fertil+Steril 2020 ; 114 (2): 233-238 Nephropedia Template TP {{tp|p=32650948|t=2020. Assessment of SARS-CoV-2 in human semen-a cohort study |pdf=|usr=}}{{32650948}} gab.com Text Assessment of SARS-CoV-2 in human semen-a cohort study JO: Fertil Steril http://www.kidney.de/pget.php?&tw=1&pmid=32650948 #FOAvip OBJECTIVE: To investigate the presence of viral RNA in human semen of patients with severe acute-respiratory syndrome coronavirus 2 (SARS-CoV-2) and to evaluate its presence and relevance in semen parameters. DESIGN: Pilot cohort study. SETTING: University hospital. PATIENT(S): Thirty-four men were distributed as: 1) patients in convalescence (patients with confirmed SARS-CoV-2 infection in pharyngeal swab according to reverse-transcription polymerase chain reaction [RT-PCR] or antibodies); 2) negative control group (no antibodies); and 3) patients with an acute infection (detection of SARS-CoV-2 in pharyngeal swab). INTERVENTION: Semen and a blood sample were collected from each individual. MAIN OUTCOME MEASURE(S): Analysis of semen quality according to the World Health Organization standards. Detection of SARS-CoV-2 by RT-PCR in the native semen sample and after density gradient preparation. Confirmation of immunoglobulin (Ig) A und IgG antibodies in the blood. RESULT(S): Eighteen semen samples from recovered men were obtained 8-54 days after absence of symptoms, 14 from control subjects, and 2 from patients with an active COVID-19 infection. No RNA was detected by means of RT-PCR in the semen, including semen samples from two patients with an acute COVID-19 infection. Subjects with a moderate infection showed an impairment of sperm quality. CONCLUSION(S): A mild COVID-19 infection is not likely to affect testis and epididymis function, whereas semen parameters did seem impaired after a moderate infection. SARS-CoV-2 RNA could not be detected in semen of recovered and acute COVID-19-positive men. This suggests no viral transmission during sexual contact and assisted reproductive techniques, although further data need to be obtained. Twit Text FOAVip Assessment of SARS-CoV-2 in human semen-a cohort study ????Fertil Steril http://www.kidney.de/pget.php?&tw=1&pmid=32650948 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32650948 #FOAvip English Wikipedia <ref>{{Cite pmid|32650948}}</ref> Assessment of SARS-CoV-2 in human semen-a cohort study #MMPMID32650948 Holtmann N; Edimiris P; Andree M; Doehmen C; Baston-Buest D; Adams O; Kruessel JS; Bielfeld AP Fertil Steril 2020[Aug]; 114 (2): 233-238 PMID32650948show ga OBJECTIVE: To investigate the presence of viral RNA in human semen of patients with severe acute-respiratory syndrome coronavirus 2 (SARS-CoV-2) and to evaluate its presence and relevance in semen parameters. DESIGN: Pilot cohort study. SETTING: University hospital. PATIENT(S): Thirty-four men were distributed as: 1) patients in convalescence (patients with confirmed SARS-CoV-2 infection in pharyngeal swab according to reverse-transcription polymerase chain reaction [RT-PCR] or antibodies); 2) negative control group (no antibodies); and 3) patients with an acute infection (detection of SARS-CoV-2 in pharyngeal swab). INTERVENTION: Semen and a blood sample were collected from each individual. MAIN OUTCOME MEASURE(S): Analysis of semen quality according to the World Health Organization standards. Detection of SARS-CoV-2 by RT-PCR in the native semen sample and after density gradient preparation. Confirmation of immunoglobulin (Ig) A und IgG antibodies in the blood. RESULT(S): Eighteen semen samples from recovered men were obtained 8-54 days after absence of symptoms, 14 from control subjects, and 2 from patients with an active COVID-19 infection. No RNA was detected by means of RT-PCR in the semen, including semen samples from two patients with an acute COVID-19 infection. Subjects with a moderate infection showed an impairment of sperm quality. CONCLUSION(S): A mild COVID-19 infection is not likely to affect testis and epididymis function, whereas semen parameters did seem impaired after a moderate infection. SARS-CoV-2 RNA could not be detected in semen of recovered and acute COVID-19-positive men. This suggests no viral transmission during sexual contact and assisted reproductive techniques, although further data need to be obtained. |Adult[MESH] |Betacoronavirus/*metabolism[MESH] |COVID-19[MESH] |Cohort Studies[MESH] |Coronavirus Infections/*blood/diagnosis[MESH] |Humans[MESH] |Infertility, Male/blood/diagnosis/virology[MESH] |Male[MESH] |Middle Aged[MESH] |Pandemics[MESH] |Pilot Projects[MESH] |Pneumonia, Viral/*blood/diagnosis[MESH] |Prospective Studies[MESH] |RNA, Viral/*blood[MESH] |SARS-CoV-2[MESH] |Semen/*metabolism/*virology[MESH]Assessment of SARS-CoV-2 in human semen-a cohort study (epmc).pdf DeepDyve Pubget Overpricing