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10.1016/j.jcv.2020.104538

http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104538
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32650276!7336953!32650276
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suck abstract from ncbi


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pmid32650276      J+Clin+Virol 2020 ; 129 (ä): 104538
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  • Clinical evaluation of the BioFire(R) Respiratory Panel 2 1 and detection of SARS-CoV-2 #MMPMID32650276
  • Creager HM; Cabrera B; Schnaubelt A; Cox JL; Cushman-Vokoun AM; Shakir SM; Tardif KD; Huang ML; Jerome KR; Greninger AL; Drobysheva D; Spaulding U; Rogatcheva M; Bourzac KM; Hinrichs SH; Broadhurst MJ; Fey PD
  • J Clin Virol 2020[Aug]; 129 (ä): 104538 PMID32650276show ga
  • We evaluated the performance of the BioFire(R) Respiratory Panel 2.1 (RP2.1) in the detection of SARS CoV-2 in comparison against three other SARS CoV-2 EUA assays. In these studies, the RP2.1 panel had 98 % positive percent agreement (48/49) and 100 % negative percent agreement (49/49). Since 30 % of nasopharyngeal swab specimens have a SARS CoV-2 Ct >30 and thus detection of virus in low titers is clinically relevant, a sample with a high titer was diluted and each 10 fold dilution was tested in triplicate and compared against 6 other EUA approved SARS CoV-2 assays. These data suggested that the BioFire(R) RP2.1 panel, along with four other SARS CoV-2 assays (Roche cobas, Cepheid Xpert Xpress, BioFire(R) Defense COVID19, and NECoV19), consistently detected viral RNA at the 10-7 dilution. Overall, these studies suggest that the BioFire(R) RP2.1 assay can be used to detect acute cases of SARS CoV2 in addition to patients with low viral titer later in disease presentation.
  • |Betacoronavirus/*isolation & purification[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques/*methods[MESH]
  • |Coronavirus Infections/*diagnosis[MESH]
  • |Humans[MESH]
  • |Molecular Diagnostic Techniques/*methods[MESH]
  • |Nasopharynx/virology[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis[MESH]
  • |Polymerase Chain Reaction/methods[MESH]
  • |RNA, Viral/*analysis/genetics[MESH]
  • |SARS-CoV-2[MESH]


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