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10.1038/s41598-020-68258-x http://scihub22266oqcxt.onion/10.1038/s41598-020-68258-x 32647249!7347621!32647249     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2020 ; 10 (1): 11323 Nephropedia Template TP {{tp|p=32647249|t=2020. Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach |pdf=|usr=}}{{32647249}} gab.com Text Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=32647249 #FOAvip Fast, cheap and easy to use nucleic acids detection methods are crucial to mitigate adverse impacts caused by various pathogens, and are essential in forensic investigations, food safety monitoring or evolution of infectious diseases. We report here a method based on the alpha-hemolysin (alpha-HL) nanopore, working in conjunction to unmodified citrate anion-coated gold nanoparticles (AuNPs), to detect nanomolar concentrations of short single-stranded DNA sequences (ssDNA). The core idea was to use charge neutral peptide nucleic acids (PNA) as hybridization probe for complementary target ssDNAs, and monitor at the single-particle level the PNA-induced aggregation propensity AuNPs during PNA-DNA duplexes formation, by recording ionic current blockades signature of AuNP-alpha-HL interactions. This approach offers advantages including: (1) a simple to operate platform, producing clear-cut readout signals based on distinct size differences of PNA-induced AuNPs aggregates, in relation to the presence in solution of complementary ssDNAs to the PNA fragments (2) sensitive and selective detection of target ssDNAs (3) specific ssDNA detection in the presence of interference DNA, without sample labeling or signal amplification. The powerful synergy of protein nanopore-based nanoparticle detection and specific PNA-DNA hybridization introduces a new strategy for nucleic acids biosensing with short detection time and label-free operation. Twit Text FOAVip Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=32647249 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32647249 #FOAvip English Wikipedia <ref>{{Cite pmid|32647249}}</ref> Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach #MMPMID32647249 Mereuta L; Asandei A; Dragomir IS; Bucataru IC; Park J; Seo CH; Park Y; Luchian T Sci Rep 2020[Jul]; 10 (1): 11323 PMID32647249show ga Fast, cheap and easy to use nucleic acids detection methods are crucial to mitigate adverse impacts caused by various pathogens, and are essential in forensic investigations, food safety monitoring or evolution of infectious diseases. We report here a method based on the alpha-hemolysin (alpha-HL) nanopore, working in conjunction to unmodified citrate anion-coated gold nanoparticles (AuNPs), to detect nanomolar concentrations of short single-stranded DNA sequences (ssDNA). The core idea was to use charge neutral peptide nucleic acids (PNA) as hybridization probe for complementary target ssDNAs, and monitor at the single-particle level the PNA-induced aggregation propensity AuNPs during PNA-DNA duplexes formation, by recording ionic current blockades signature of AuNP-alpha-HL interactions. This approach offers advantages including: (1) a simple to operate platform, producing clear-cut readout signals based on distinct size differences of PNA-induced AuNPs aggregates, in relation to the presence in solution of complementary ssDNAs to the PNA fragments (2) sensitive and selective detection of target ssDNAs (3) specific ssDNA detection in the presence of interference DNA, without sample labeling or signal amplification. The powerful synergy of protein nanopore-based nanoparticle detection and specific PNA-DNA hybridization introduces a new strategy for nucleic acids biosensing with short detection time and label-free operation. |Biosensing Techniques/*methods[MESH] |DNA Probes[MESH] |DNA, Single-Stranded/*isolation & purification[MESH] |Gold/chemistry[MESH] |Hemolysin Proteins/chemistry[MESH] |Metal Nanoparticles/*chemistry[MESH] |Nanopores[MESH] |Nucleic Acid Hybridization/*methods[MESH]Sequence-specific detection of single-stranded DNA with a gold nanopa(epmc).pdf DeepDyve Pubget Overpricing