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10.3390/v12070735 http://scihub22266oqcxt.onion/10.3390/v12070735 32646015!7412566!32646015     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2020 ; 12 (7): ä Nephropedia Template TP {{tp|p=32646015|t=2020. Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics?|pdf=|usr=}}{{32646015}} gab.com Text Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32646015 #FOAvip Standard precautions to minimize the risk of SARS-CoV-2 transmission implies that infected cell cultures and clinical specimens may undergo some sort of inactivation to reduce or abolish infectivity. We evaluated three heat inactivation protocols (56 degrees C-30 min, 60 degrees C-60 min and 92 degrees C-15 min) on SARS-CoV-2 using (i) infected cell culture supernatant, (ii) virus-spiked human sera (iii) and nasopharyngeal samples according to the recommendations of the European norm NF EN 14476-A2. Regardless of the protocol and the type of samples, a 4 Log(10) TCID50 reduction was observed. However, samples containing viral loads > 6 Log(10) TCID(50) were still infectious after 56 degrees C-30 min and 60 degrees C-60 min, although infectivity was < 10 TCID(50). The protocols 56 degrees C-30 min and 60 degrees C-60 min had little influence on the RNA copies detection, whereas 92 degrees C-15 min drastically reduced the limit of detection, which suggests that this protocol should be avoided for inactivation ahead of molecular diagnostics. Lastly, 56 degrees C-30 min treatment of serum specimens had a negligible influence on the results of IgG detection using a commercial ELISA test, whereas a drastic decrease in neutralizing titers was observed. Twit Text FOAVip Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32646015 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32646015 #FOAvip English Wikipedia <ref>{{Cite pmid|32646015}}</ref> Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics? #MMPMID32646015 Pastorino B; Touret F; Gilles M; de Lamballerie X; Charrel RN Viruses 2020[Jul]; 12 (7): ä PMID32646015show ga Standard precautions to minimize the risk of SARS-CoV-2 transmission implies that infected cell cultures and clinical specimens may undergo some sort of inactivation to reduce or abolish infectivity. We evaluated three heat inactivation protocols (56 degrees C-30 min, 60 degrees C-60 min and 92 degrees C-15 min) on SARS-CoV-2 using (i) infected cell culture supernatant, (ii) virus-spiked human sera (iii) and nasopharyngeal samples according to the recommendations of the European norm NF EN 14476-A2. Regardless of the protocol and the type of samples, a 4 Log(10) TCID50 reduction was observed. However, samples containing viral loads > 6 Log(10) TCID(50) were still infectious after 56 degrees C-30 min and 60 degrees C-60 min, although infectivity was < 10 TCID(50). The protocols 56 degrees C-30 min and 60 degrees C-60 min had little influence on the RNA copies detection, whereas 92 degrees C-15 min drastically reduced the limit of detection, which suggests that this protocol should be avoided for inactivation ahead of molecular diagnostics. Lastly, 56 degrees C-30 min treatment of serum specimens had a negligible influence on the results of IgG detection using a commercial ELISA test, whereas a drastic decrease in neutralizing titers was observed. |*Betacoronavirus/immunology[MESH] |*Virus Inactivation[MESH] |Antibodies, Neutralizing/immunology[MESH] |COVID-19[MESH] |Containment of Biohazards/*methods/standards[MESH] |Coronavirus Infections/diagnosis/prevention & control/*virology[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Hot Temperature[MESH] |Humans[MESH] |Neutralization Tests[MESH] |Pandemics/prevention & control[MESH] |Pneumonia, Viral/diagnosis/prevention & control/*virology[MESH] |SARS-CoV-2[MESH]Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Proto(epmc).pdf DeepDyve Pubget Overpricing