Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.3390/ijms21134807 http://scihub22266oqcxt.onion/10.3390/ijms21134807 32645951!7370282!32645951     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Sci 2020 ; 21 (13): ä Nephropedia Template TP {{tp|p=32645951|t=2020. Implications of SARS-CoV-2 Mutations for Genomic RNA Structure and Host microRNA Targeting |pdf=|usr=}}{{32645951}} gab.com Text Implications of SARS-CoV-2 Mutations for Genomic RNA Structure and Host microRNA Targeting JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=32645951 #FOAvip The SARS-CoV-2 virus is a recently-emerged zoonotic pathogen already well adapted to transmission and replication in humans. Although the mutation rate is limited, recently introduced mutations in SARS-CoV-2 have the potential to alter viral fitness. In addition to amino acid changes, mutations could affect RNA secondary structure critical to viral life cycle, or interfere with sequences targeted by host miRNAs. We have analysed subsets of genomes from SARS-CoV-2 isolates from around the globe and show that several mutations introduce changes in Watson-Crick pairing, with resultant changes in predicted secondary structure. Filtering to targets matching miRNAs expressed in SARS-CoV-2-permissive host cells, we identified ten separate target sequences in the SARS-CoV-2 genome; three of these targets have been lost through conserved mutations. A genomic site targeted by the highly abundant miR-197-5p, overexpressed in patients with cardiovascular disease, is lost by a conserved mutation. Our results are compatible with a model that SARS-CoV-2 replication within the human host is constrained by host miRNA defences. The impact of these and further mutations on secondary structures, miRNA targets or potential splice sites offers a new context in which to view future SARS-CoV-2 evolution, and a potential platform for engineering conditional attenuation to vaccine development, as well as providing a better understanding of viral tropism and pathogenesis. Twit Text FOAVip Implications of SARS-CoV-2 Mutations for Genomic RNA Structure and Host microRNA Targeting ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=32645951 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32645951 #FOAvip English Wikipedia <ref>{{Cite pmid|32645951}}</ref> Implications of SARS-CoV-2 Mutations for Genomic RNA Structure and Host microRNA Targeting #MMPMID32645951 Hosseini Rad Sm A; McLellan AD Int J Mol Sci 2020[Jul]; 21 (13): ä PMID32645951show ga The SARS-CoV-2 virus is a recently-emerged zoonotic pathogen already well adapted to transmission and replication in humans. Although the mutation rate is limited, recently introduced mutations in SARS-CoV-2 have the potential to alter viral fitness. In addition to amino acid changes, mutations could affect RNA secondary structure critical to viral life cycle, or interfere with sequences targeted by host miRNAs. We have analysed subsets of genomes from SARS-CoV-2 isolates from around the globe and show that several mutations introduce changes in Watson-Crick pairing, with resultant changes in predicted secondary structure. Filtering to targets matching miRNAs expressed in SARS-CoV-2-permissive host cells, we identified ten separate target sequences in the SARS-CoV-2 genome; three of these targets have been lost through conserved mutations. A genomic site targeted by the highly abundant miR-197-5p, overexpressed in patients with cardiovascular disease, is lost by a conserved mutation. Our results are compatible with a model that SARS-CoV-2 replication within the human host is constrained by host miRNA defences. The impact of these and further mutations on secondary structures, miRNA targets or potential splice sites offers a new context in which to view future SARS-CoV-2 evolution, and a potential platform for engineering conditional attenuation to vaccine development, as well as providing a better understanding of viral tropism and pathogenesis. |*Genome, Viral[MESH] |3' Untranslated Regions[MESH] |Base Sequence[MESH] |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |Coronavirus Infections/pathology/virology[MESH] |Databases, Genetic[MESH] |Humans[MESH] |MicroRNAs/chemistry/genetics/*metabolism[MESH] |Mutation[MESH] |Nucleic Acid Conformation[MESH] |Pandemics[MESH] |Pneumonia, Viral/pathology/virology[MESH] |RNA Splice Sites[MESH] |RNA Splicing[MESH] |RNA, Viral/*chemistry[MESH] |SARS-CoV-2[MESH] |Sequence Alignment[MESH] |Viral Nonstructural Proteins/genetics[MESH]Implications of SARS-CoV-2 Mutations for Genomic RNA Structure and Hos(epmc).pdf DeepDyve Pubget Overpricing