Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Cell 2020 ; 182 (3): 734-743.e5 Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Generation of a Broadly Useful Model for COVID-19 Pathogenesis, Vaccination, and Treatment #MMPMID32643603
Sun J; Zhuang Z; Zheng J; Li K; Wong RL; Liu D; Huang J; He J; Zhu A; Zhao J; Li X; Xi Y; Chen R; Alshukairi AN; Chen Z; Zhang Z; Chen C; Huang X; Li F; Lai X; Chen D; Wen L; Zhuo J; Zhang Y; Wang Y; Huang S; Dai J; Shi Y; Zheng K; Leidinger MR; Chen J; Li Y; Zhong N; Meyerholz DK; McCray PB Jr; Perlman S; Zhao J
Cell 2020[Aug]; 182 (3): 734-743.e5 PMID32643603show ga
COVID-19, caused by SARS-CoV-2, is a virulent pneumonia, with >4,000,000 confirmed cases worldwide and >290,000 deaths as of May 15, 2020. It is critical that vaccines and therapeutics be developed very rapidly. Mice, the ideal animal for assessing such interventions, are resistant to SARS-CoV-2. Here, we overcome this difficulty by exogenous delivery of human ACE2 with a replication-deficient adenovirus (Ad5-hACE2). Ad5-hACE2-sensitized mice developed pneumonia characterized by weight loss, severe pulmonary pathology, and high-titer virus replication in lungs. Type I interferon, T cells, and, most importantly, signal transducer and activator of transcription 1 (STAT1) are critical for virus clearance and disease resolution in these mice. Ad5-hACE2-transduced mice enabled rapid assessments of a vaccine candidate, of human convalescent plasma, and of two antiviral therapies (poly I:C and remdesivir). In summary, we describe a murine model of broad and immediate utility to investigate COVID-19 pathogenesis and to evaluate new therapies and vaccines.