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10.1093/cid/ciaa941 http://scihub22266oqcxt.onion/10.1093/cid/ciaa941 32642757!7454436!32642757     free     free     free       Clin+Infect+Dis 2021 ; 73 (2): e426-e433 Nephropedia Template TP {{tp|p=32642757|t=2021. A Confirmed Case of SARS-CoV-2 Pneumonia With Negative Routine Reverse Transcriptase-Polymerase Chain Reaction and Virus Variation in Guangzhou, China |pdf=|usr=}}{{32642757}} gab.com Text A Confirmed Case of SARS-CoV-2 Pneumonia With Negative Routine Reverse Transcriptase-Polymerase Chain Reaction and Virus Variation in Guangzhou, China JO: Clin Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32642757 #FOAvip BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine reverse transcriptase -polymerase chain reaction (RT-PCR) detection of SARS-CoV-2. METHODS: However, we report a confirmed case of SARS-CoV-2 pneumonia with a negative routine RT-PCR. RESULTS: This case was finally diagnosed by nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variations of SARS-CoV-2 were found. CONCLUSIONS: This case highlighted that false-negative results could be present in routine RT-PCR diagnosis, especially with virus variation. Currently, nanopore pathogen sequencing and antibody detection have been found to be effective in clinical diagnosis. Twit Text FOAVip A Confirmed Case of SARS-CoV-2 Pneumonia With Negative Routine Reverse Transcriptase-Polymerase Chain Reaction and Virus Variation in Guangzhou, China ????Clin Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32642757 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32642757 #FOAvip English Wikipedia <ref>{{Cite pmid|32642757}}</ref> A Confirmed Case of SARS-CoV-2 Pneumonia With Negative Routine Reverse Transcriptase-Polymerase Chain Reaction and Virus Variation in Guangzhou, China #MMPMID32642757 Li Z; Li Y; Chen L; Li S; Yu L; Zhu A; Yang F; Jiang Q; Chen L; Zhao J; Lu W; Zhong N; Ye F Clin Infect Dis 2021[Jul]; 73 (2): e426-e433 PMID32642757show ga BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine reverse transcriptase -polymerase chain reaction (RT-PCR) detection of SARS-CoV-2. METHODS: However, we report a confirmed case of SARS-CoV-2 pneumonia with a negative routine RT-PCR. RESULTS: This case was finally diagnosed by nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variations of SARS-CoV-2 were found. CONCLUSIONS: This case highlighted that false-negative results could be present in routine RT-PCR diagnosis, especially with virus variation. Currently, nanopore pathogen sequencing and antibody detection have been found to be effective in clinical diagnosis. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |China[MESH] |Humans[MESH] |RNA-Directed DNA Polymerase[MESH]A Confirmed Case of SARS-CoV-2 Pneumonia With Negative Routine Reverse(epmc).pdf DeepDyve Pubget Overpricing