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10.7150/thno.47649 http://scihub22266oqcxt.onion/10.7150/thno.47649 32641984!7330846!32641984     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Theranostics 2020 ; 10 (16): 7150-7162 Nephropedia Template TP {{tp|p=32641984|t=2020. Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2 |pdf=|usr=}}{{32641984}} gab.com Text Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2 JO: Theranostics http://www.kidney.de/pget.php?&tw=1&pmid=32641984 #FOAvip In December 2019, a new coronavirus disease (COVID-19) outbreak occurred in Wuhan, China. Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which is the seventh coronavirus known to infect humans, is highly contagious and has rapidly expanded worldwide since its discovery. Quantitative nucleic acid testing has become the gold standard for diagnosis and guiding clinical decisions regarding the use of antiviral therapy. However, the RT-qPCR assays targeting SARS-CoV-2 have a number of challenges, especially in terms of primer design. Primers are the pivotal components of a RT-qPCR assay. Once virus mutation and recombination occur, it is difficult to effectively diagnose viral infection by existing RT-qPCR primers. Some primers and probes have also been made available on the WHO website for reference. However, no previous review has systematically compared the previously reported primers and probes and described how to design new primers in the event of a new coronavirus infection. This review focuses on how primers and probes can be designed methodically and rationally, and how the sensitivity and specificity of the detection process can be improved. This brief review will be useful for the accurate diagnosis and timely treatment of the new coronavirus pneumonia. Twit Text FOAVip Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2 ????Theranostics http://www.kidney.de/pget.php?&tw=1&pmid=32641984 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32641984 #FOAvip English Wikipedia <ref>{{Cite pmid|32641984}}</ref> Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2 #MMPMID32641984 Li D; Zhang J; Li J Theranostics 2020[]; 10 (16): 7150-7162 PMID32641984show ga In December 2019, a new coronavirus disease (COVID-19) outbreak occurred in Wuhan, China. Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which is the seventh coronavirus known to infect humans, is highly contagious and has rapidly expanded worldwide since its discovery. Quantitative nucleic acid testing has become the gold standard for diagnosis and guiding clinical decisions regarding the use of antiviral therapy. However, the RT-qPCR assays targeting SARS-CoV-2 have a number of challenges, especially in terms of primer design. Primers are the pivotal components of a RT-qPCR assay. Once virus mutation and recombination occur, it is difficult to effectively diagnose viral infection by existing RT-qPCR primers. Some primers and probes have also been made available on the WHO website for reference. However, no previous review has systematically compared the previously reported primers and probes and described how to design new primers in the event of a new coronavirus infection. This review focuses on how primers and probes can be designed methodically and rationally, and how the sensitivity and specificity of the detection process can be improved. This brief review will be useful for the accurate diagnosis and timely treatment of the new coronavirus pneumonia. |Base Sequence[MESH] |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis/epidemiology/virology[MESH] |Drug Design[MESH] |Genes, Viral[MESH] |Humans[MESH] |Nucleic Acid Conformation[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/epidemiology/virology[MESH] |RNA Probes/genetics[MESH] |RNA, Viral/chemistry/*genetics[MESH] |RNA/chemistry/*genetics[MESH] |Real-Time Polymerase Chain Reaction/*methods/statistics & numerical data[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH]Primer design for quantitative real-time PCR for the emerging Coronavi(epmc).pdf DeepDyve Pubget Overpricing