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10.2144/btn-2020-0078 http://scihub22266oqcxt.onion/10.2144/btn-2020-0078 32635743!ä!32635743 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biotechniques 2020 ; 69 (3): 178-185 Nephropedia Template TP {{tp|p=32635743|t=2020. Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride |pdf=|usr=}}{{32635743}} gab.com Text Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride JO: Biotechniques http://www.kidney.de/pget.php?&tw=1&pmid=32635743 #FOAvip Loop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. The global SARS-CoV-2 pandemic has presented an urgent need for new and better diagnostic methods, with colorimetric LAMP utilized in numerous studies for SARS-CoV-2 detection. However, the sensitivity of colorimetric LAMP in early reports has been below that of the standard RT-qPCR tests, and we sought to improve performance. Here we report the use of guanidine hydrochloride and combined primer sets to increase speed and sensitivity in colorimetric LAMP, bringing this simple method up to the standards of sophisticated techniques and enabling accurate, high-throughput diagnostics. Twit Text FOAVip Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride ????Biotechniques http://www.kidney.de/pget.php?&tw=1&pmid=32635743 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32635743 #FOAvip English Wikipedia <ref>{{Cite pmid|32635743}}</ref> Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride #MMPMID32635743 Zhang Y; Ren G; Buss J; Barry AJ; Patton GC; Tanner NA Biotechniques 2020[Sep]; 69 (3): 178-185 PMID32635743show ga Loop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. The global SARS-CoV-2 pandemic has presented an urgent need for new and better diagnostic methods, with colorimetric LAMP utilized in numerous studies for SARS-CoV-2 detection. However, the sensitivity of colorimetric LAMP in early reports has been below that of the standard RT-qPCR tests, and we sought to improve performance. Here we report the use of guanidine hydrochloride and combined primer sets to increase speed and sensitivity in colorimetric LAMP, bringing this simple method up to the standards of sophisticated techniques and enabling accurate, high-throughput diagnostics. |*Guanidine[MESH] |Betacoronavirus/*isolation & purification[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/*methods/standards[MESH] |Colorimetry[MESH] |Coronavirus Infections/diagnosis[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/*methods/standards[MESH] |Nucleic Acid Amplification Techniques/*methods/standards[MESH] |Phenolsulfonphthalein[MESH]Enhancing colorimetric loop-mediated isothermal amplification speed an(epmc).pdf DeepDyve Pubget Overpricing