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10.1016/j.meegid.2020.104443

http://scihub22266oqcxt.onion/10.1016/j.meegid.2020.104443
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suck abstract from ncbi


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pmid32619637      Infect+Genet+Evol 2020 ; 85 (ä): 104443
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  • Immune responses and protective effects against Japanese encephalitis induced by a DNA vaccine encoding the prM/E proteins of the attenuated SA14-14-2 strain #MMPMID32619637
  • Zheng X; Yu X; Wang Y; Cui M; Wang R; Yin C
  • Infect Genet Evol 2020[Nov]; 85 (ä): 104443 PMID32619637show ga
  • Japanese encephalitis virus (JEV) is the causal pathogen of Japanese encephalitis (JE), which has become a severe public health problem and is one of the most rapidly spreading mosquito-borne diseases worldwide. Currently, there is no specific treatment for JEV. A vaccine would be an effective measure for reducing morbidity and mortality. Although the live attenuated vaccine SA14-14-2 has been approved in some countries, it is still necessary to develop safer, more effective, and less costly vaccines. In this study, a DNA vaccine candidate, pV-SA14ME, expressing the prM/E proteins of SA14-14-2 was inoculated into BALB/c mice via intramuscular electroporation, and the immunogenicity and degree of protection were evaluated. We found that administration of 50 mug pV-SA14ME via electroporation via three immunizations could induce persistent humoral and cellular immune responses and effectively protect mice against lethal JEV challenge. This study provides a basis for the subsequent promotion and use of the vaccine and lays the foundation for its further use in swine and humans.
  • |*Immunity[MESH]
  • |Animals[MESH]
  • |Antibodies, Viral/metabolism[MESH]
  • |Encephalitis Virus, Japanese/genetics/*immunology[MESH]
  • |Encephalitis, Japanese/*immunology/virology[MESH]
  • |Female[MESH]
  • |HEK293 Cells[MESH]
  • |Humans[MESH]
  • |Mice, Inbred BALB C[MESH]
  • |Vaccination[MESH]
  • |Vaccines, Attenuated[MESH]
  • |Vaccines, DNA/*genetics/*immunology/virology[MESH]


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