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10.1080/22221751.2020.1791737 http://scihub22266oqcxt.onion/10.1080/22221751.2020.1791737 32619390!7473198!32619390     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32619390&cmd=llinks): Failed to open stream: HTTP request failed! 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Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines |pdf=|usr=}}{{32619390}} gab.com Text Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines JO: Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32619390 #FOAvip Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and is continuing to spread rapidly around the globe. No effective vaccine is currently available to prevent COVID-19, and intense efforts are being invested worldwide into vaccine development. In this context, all technology platforms must overcome several challenges resulting from the use of an incompletely characterized new virus. These include finding the right conditions for virus amplification for the development of vaccines based on inactivated or attenuated whole viral particles. Here, we describe a shotgun tandem mass spectrometry workflow, the data produced can be used to guide optimization of the conditions for viral amplification. In parallel, we analysed the changes occurring in the host cell proteome following SARS-CoV-2 infection to glean information on the biological processes modulated by the virus that could be further explored as potential drug targets to deal with the pandemic. Twit Text FOAVip Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines ????Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32619390 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32619390 #FOAvip English Wikipedia <ref>{{Cite pmid|32619390}}</ref> Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines #MMPMID32619390 Grenga L; Gallais F; Pible O; Gaillard JC; Gouveia D; Batina H; Bazaline N; Ruat S; Culotta K; Miotello G; Debroas S; Roncato MA; Steinmetz G; Foissard C; Desplan A; Alpha-Bazin B; Almunia C; Gas F; Bellanger L; Armengaud J Emerg Microbes Infect 2020[Dec]; 9 (1): 1712-1721 PMID32619390show ga Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and is continuing to spread rapidly around the globe. No effective vaccine is currently available to prevent COVID-19, and intense efforts are being invested worldwide into vaccine development. In this context, all technology platforms must overcome several challenges resulting from the use of an incompletely characterized new virus. These include finding the right conditions for virus amplification for the development of vaccines based on inactivated or attenuated whole viral particles. Here, we describe a shotgun tandem mass spectrometry workflow, the data produced can be used to guide optimization of the conditions for viral amplification. In parallel, we analysed the changes occurring in the host cell proteome following SARS-CoV-2 infection to glean information on the biological processes modulated by the virus that could be further explored as potential drug targets to deal with the pandemic. |Animals[MESH] |Antigens, Viral/*biosynthesis/immunology[MESH] |Betacoronavirus/*immunology[MESH] |Chlorocebus aethiops[MESH] |Proteomics/*methods[MESH] |SARS-CoV-2[MESH] |Tandem Mass Spectrometry[MESH] |Vero Cells[MESH] |Viral Vaccines/*immunology[MESH]Shotgun proteomics analysis of SARS-CoV-2-infected cells and how it ca(epmc).pdf DeepDyve Pubget Overpricing