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10.7883/yoken.JJID.2020.324

http://scihub22266oqcxt.onion/10.7883/yoken.JJID.2020.324
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32611983!ä!32611983

suck abstract from ncbi


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pmid32611983      Jpn+J+Infect+Dis 2021 ; 74 (1): 29-34
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  • An Ultra-Rapid Real-Time RT-PCR Method Using the PCR1100 to Detect Severe Acute Respiratory Syndrome Coronavirus-2 #MMPMID32611983
  • Shirato K; Nao N; Matsuyama S; Takeda M; Kageyama T
  • Jpn J Infect Dis 2021[Jan]; 74 (1): 29-34 PMID32611983show ga
  • The disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in Wuhan, China, in December 2019, has rapidly spread worldwide. SARS-CoV-2 is usually detected via real-time reverse-transcription polymerase chain reaction (RT-PCR). However, the increase in specimen load in institutions/hospitals necessitates a simpler detection system. Here, we present an ultra-rapid, real-time RT-PCR assay for SARS-CoV-2 detection using PCR1100 device. Although PCR1100 tests only one specimen at a time, the amplification period is less than 20 min and the sensitivity and specificity match those of conventional real-time RT-PCR performed on large instruments. The method is potentially helpful when daily multiple SARS-CoV-2 testing is needed, for example to confirm virus-free status prior to patient discharge.
  • |COVID-19 Testing/*instrumentation/methods[MESH]
  • |COVID-19/*virology[MESH]
  • |Humans[MESH]
  • |Real-Time Polymerase Chain Reaction/*instrumentation/methods[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction/*instrumentation/methods[MESH]
  • |SARS-CoV-2/genetics/*isolation & purification[MESH]


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