Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2 #MMPMID32610037
Paden CR; Tao Y; Queen K; Zhang J; Li Y; Uehara A; Tong S
Emerg Infect Dis 2020[Oct]; 26 (10): 2401-2405 PMID32610037show ga
We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.