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Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Emerg+Infect+Dis 2020 ; 26 (10): 2401-2405 Nephropedia Template TP
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Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2 #MMPMID32610037
Paden CR; Tao Y; Queen K; Zhang J; Li Y; Uehara A; Tong S
Emerg Infect Dis 2020[Oct]; 26 (10): 2401-2405 PMID32610037show ga
We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.